Fidler E, Ansari A
Department of Biological Science, Wayne State University, USA.
Int J Biochem Physiol. 2024;9(1). doi: 10.23880/ijbp-16000250. Epub 2024 Jun 6.
The generally accepted view is that different steps of transcription, mRNA processing and mRNA trafficking are performed by dedicated accessory factors. The research during last couple of decades, however, has challenged this dogma. We recently showed that the general transcription factor TFIIB, in addition to its well established role in initiation, is also involved in termination of transcription. Mass spectrometry of purified TFIIB revealed Sub2 as one of its major interacting partners. Sub2 has already been implicated in elongation step of transcription, splicing and mRNA trafficking. Interaction of Sub2 with TFIIB implicates it in initiation and termination steps of transcription. Multiplicity of Sub2 biological roles may be responsible for its link with a number of human pathological conditions making it a potential drug target.
普遍接受的观点是，转录、mRNA加工和mRNA运输的不同步骤由专门的辅助因子执行。然而，过去几十年的研究对这一教条提出了挑战。我们最近发现，通用转录因子TFIIB除了在起始阶段发挥其已确立的作用外，还参与转录的终止。对纯化的TFIIB进行质谱分析发现，Sub2是其主要相互作用伙伴之一。Sub2已被证明参与转录的延伸步骤、剪接和mRNA运输。Sub2与TFIIB的相互作用表明它参与转录的起始和终止步骤。Sub2生物学作用的多样性可能是其与多种人类病理状况相关的原因，使其成为一个潜在的药物靶点。
