通过RNA干扰介导的敲低，褐飞虱雄性性腺发育和精子活力降低 。 （注：原文中“Knockdown of ”后面缺少具体内容）
Reduction in Gonad Development and Sperm Motility in Male Brown Planthopper via RNAi-Mediated Knockdown of .
作者信息
Feng Bo, Hu Yang, Wang Fanghai
机构信息
State Key Laboratory for Biocontrol and Institute of Entomology, Sun Yat-sen University, Guangzhou 510275, China.
出版信息
Int J Mol Sci. 2025 Apr 12;26(8):3643. doi: 10.3390/ijms26083643.
The brown planthopper , a major rice pest, threatens global food security through rapid reproduction. This study investigates the role of the () gene in male reproductive development and spermatogenesis using RNA interference (RNAi). Gene expression analysis revealed higher levels in testes. RNAi-mediated knockdown of in fourth-instar male nymphs reduced its expression by up to 80%, leading to severely impaired gonad development. Testes, vas deferens, and accessory glands in treated males exhibited 8-89% volume reductions compared to controls, accompanied by a 51-69% decline in sperm count and 60-85% reduction in sperm motility. Consequently, eggs fertilized by treated males showed a 73% decrease in hatching rates, with arrested embryonic development. These findings demonstrate 's critical role in spermatogenesis and gonad maturation in , highlighting its potential as an RNAi target for sustainable pest control strategies.
褐飞虱是一种主要的水稻害虫，通过快速繁殖威胁全球粮食安全。本研究利用RNA干扰（RNAi）技术研究了（）基因在雄性生殖发育和精子发生中的作用。基因表达分析显示睾丸中该基因水平较高。对四龄雄性若虫进行RNAi介导的该基因敲低，使其表达降低了80%，导致性腺发育严重受损。与对照组相比，处理后的雄性个体的睾丸、输精管和附腺体积减少了8%-89%，同时精子数量下降了51%-69%，精子活力降低了60%-85%。因此，经处理的雄性个体受精的卵孵化率下降了73%，胚胎发育停滞。这些发现证明了该基因在褐飞虱精子发生和性腺成熟中的关键作用，突出了其作为可持续害虫控制策略的RNAi靶点的潜力。
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