Zhou Zuying, Zhou Yang, Wang Pu, Zhou Ting, Chi Mingyan, Li Yueting, Zhou Meng, Yang Shuai, Wang Aimin, Zheng Lin, Huang Yong
State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang 550004, China.
School of Pharmaceutical Sciences, Guizhou Medical University, 4 Beijing Road, Guiyang 550004, China.
Int J Mol Sci. 2025 Apr 14;26(8):3694. doi: 10.3390/ijms26083694.
(Roth) O. Deg. (Fabaceae), traditionally used by the Hmong people to treat rheumatoid arthritis (RA), has not been extensively studied for the correlation between its anti-inflammatory activity and its active components. Protosappanoside D (PTD), a new component, has been isolated for the first time from the extract of . This study focused on the anti-arthritic and anti-inflammatory effects of extracts (BDE) and PTD, along with their pharmacokinetic-pharmacodynamic (PK-PD) analysis. In the adjuvant-induced arthritis (AA) rat model, HE staining and cytokine assays showed that BDE alleviated joint damage and reduced inflammatory cytokines, similar to the positive control. In the LPS-induced inflammatory cell model, both BDE and PTD demonstrated anti-inflammatory effects by inhibiting the secretion of inflammatory factors. A PK-PD analysis of BDE in AA rats and inflammatory cells, as well as an analysis of PTD as a monomer, was conducted. The results indicated that PTD had different regulatory effects on cytokines like TNF-, with a certain lag and sustained effects. These findings suggest the potential of BDE and PTD as treatments for rheumatoid arthritis, though further in vivo studies and clinical trials are needed.
（豆科）刺果苏木，传统上被苗族用于治疗类风湿性关节炎（RA），但其抗炎活性与其活性成分之间的相关性尚未得到广泛研究。原苏木皂苷D（PTD），一种新成分，首次从其提取物中分离出来。本研究聚焦于刺果苏木提取物（BDE）和PTD的抗关节炎和抗炎作用，以及它们的药代动力学-药效学（PK-PD）分析。在佐剂性关节炎（AA）大鼠模型中，苏木精-伊红（HE）染色和细胞因子检测表明，BDE减轻了关节损伤并降低了炎性细胞因子，与阳性对照相似。在脂多糖（LPS）诱导的炎症细胞模型中，BDE和PTD均通过抑制炎性因子的分泌表现出抗炎作用。对AA大鼠和炎症细胞中的BDE进行了PK-PD分析，并对作为单体的PTD进行了分析。结果表明，PTD对肿瘤坏死因子-α（TNF-α）等细胞因子具有不同的调节作用，具有一定的滞后性和持续效应。这些发现表明BDE和PTD作为类风湿性关节炎治疗药物的潜力，不过还需要进一步的体内研究和临床试验。
