Bai Yu, Ouyang Huizi, Liu Yang, Zuo Fanjiao, Li Caixia, Zhou Shuting, Chang Yanxu, He Jun
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China.
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, 300193, Tianjin, China.
Curr Drug Metab. 2025;25(8):576-585. doi: 10.2174/0113892002301262241107065717.
Cnidii Fructus (CF) is a herbal medicine with pharmacological activities such as antitumor, antiviral, antiallergic, antipruritic effects, and so on.
In this study, an ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC- MS/MS) method was prepared and verified to measure the concentrations of seven analytes (bergapten, xanthotoxol, xanthotoxin, imperatorin, osthole, isopimpinellin, isoimperatorin) in HepG2 cells.
The separation of seven analytes was performed on an ACQUITY UPLC® BEH C18 column (2.1×100 mm, 1.7 μm) with a gradient mobile phase system of 0.1% formic acid/water and acetonitrile.
The CV of analytes was within 7.77%, and the bias was in the range of -5.43%-3.84%. The matrix effects of analytes ranged from 92.95% to 104.58%, and the extraction recoveries ranged from 76.45% to 104.69%. The relative standard deviation of stability results was less than 8.21%, indicating that seven analytes were stable.
The method was successfully applied to the determination of the content of seven analytes of CF extracts by UPLC-MS/MS, and the results will provide a reference for the cellular pharmacokinetics of CF.
蛇床子是一种具有抗肿瘤、抗病毒、抗过敏、止痒等药理活性的草药。
本研究建立并验证了一种超高效液相色谱/串联质谱（UPLC-MS/MS）方法，用于测定HepG2细胞中七种分析物（补骨脂素、花椒毒素、异补骨脂素、欧前胡素、蛇床子素、异茴芹内酯、异欧前胡素）的浓度。
在ACQUITY UPLC® BEH C18柱（2.1×100 mm，1.7 μm）上，采用0.1%甲酸/水和乙腈的梯度流动相系统对七种分析物进行分离。
分析物的变异系数在7.77%以内，偏差在-5.43%至3.84%范围内。分析物的基质效应在92.95%至104.58%之间，提取回收率在76.45%至104.69%之间。稳定性结果的相对标准偏差小于8.21%，表明七种分析物稳定。
该方法成功应用于UPLC-MS/MS测定蛇床子提取物中七种分析物的含量，结果将为蛇床子的细胞药代动力学提供参考。
