Zhao Yuge, Wang Chao, Liu Xinmeng, Lin Xu, Chu Dongdong, Ding Junyi, Kong Xiangbo, Chen Dafeng
State Forestry and Grassland Administration Key Laboratory of Silviculture in Downstream Areas of the Yellow River, College of Forestry, Shandong Agricultural University, Tai'an 271018, China.
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
Insects. 2025 Apr 3;16(4):384. doi: 10.3390/insects16040384.
The Asian larch bark beetle, Motschulsky, is a severe pest of various species in its natural range. This study reports the degree of similarity among cuticular hydrocarbon (CHC) profiles of six populations of in northeastern China. Thirty individual or isomeric mixtures of hydrocarbons were identified by solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS). The hydrocarbon components consist of straight-chain alkanes, alkenes, and methyl-branched hydrocarbons with carbon chain lengths ranging from 24 to 31. Among these, four CHCs (-C, 9-C, -C, and 3-meC) can serve as chemotaxonomic markers to identify . No qualitative differences were detected between males and females in any of the six populations, but significant quantitative differences were observed in some CHCs. Phylogenetic analysis based on CHC profiles showed only minor differences compared to analysis based on partial mtDNA COI sequences regarding bark beetle species affinity. These results establish a rapid chemotaxonomic method and provide a basis for further investigations into the functions of CHCs in .
亚洲落叶松八齿小蠹（Motschulsky）是其天然分布范围内多种树木的严重害虫。本研究报告了中国东北地区六个亚洲落叶松八齿小蠹种群表皮碳氢化合物（CHC）谱的相似程度。通过固相微萃取（SPME）结合气相色谱-质谱联用（GC-MS）鉴定出30种碳氢化合物的单一成分或异构体混合物。碳氢化合物成分包括直链烷烃、烯烃和碳链长度为24至31的甲基支链碳氢化合物。其中，四种CHC（-C、9-C、-C和3-meC）可作为化学分类标记物来鉴定亚洲落叶松八齿小蠹。六个种群中的任何一个种群的雌雄个体之间均未检测到定性差异，但在某些CHC中观察到了显著的定量差异。基于CHC谱的系统发育分析显示，与基于部分线粒体DNA COI序列的分析相比，在小蠹虫物种亲缘关系方面仅存在微小差异。这些结果建立了一种快速的化学分类方法，并为进一步研究亚洲落叶松八齿小蠹中CHC的功能提供了基础。
