克罗恩病中针对人类内源性逆转录病毒的体液免疫反应的特征

A Characterization of the Humoral Immune Response to Human Endogenous Retroviruses and in Crohn's Disease.

作者信息

Fayyaz Alishba, Cugia Luigi, Noli Marta, Jasemi Somaye, Simula Elena Rita, Sechi Leonardo A

机构信息

Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy.

Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy.

出版信息

Pathogens. 2025 Apr 7;14(4):361. doi: 10.3390/pathogens14040361.

DOI:10.3390/pathogens14040361

PMID:40333136

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12030244/

Abstract

Crohn's disease (CD) is a multifactorial polygenic inflammatory bowel disease linked to aberrant immune response. (MAP) has been associated with CD; however, detecting MAP in CD tissues remains highly challenging. Recently, Human Endogenous Retroviruses (HERVs) differential gene expression has been reported in CD, but little is known about the involvement of MAP and HERVs in CD pathology. This study aimed to characterize the humoral response against HERV-K, HERV-W, and MAP antigens using an indirect ELISA in plasma samples from CD patients and age- and gender-matched healthy controls (HCs). We observed a significant antibody response against HERV-K and HERV-W epitopes in CD patients in comparison to MAP epitopes, as well as a higher overall antibody response in patients compared to HCs. This study is the first to report the presence of humoral immune response against HERVs antigens in CD. Considering the pro-inflammatory nature of CD, HERVs may contribute to the development or progression of disease in genetically predisposed individuals. However, further research is needed to better understand the complex role of HERVs in CD.

摘要

克罗恩病（CD）是一种与异常免疫反应相关的多因素多基因炎症性肠病。鸟分枝杆菌副结核亚种（MAP）与CD有关；然而，在CD组织中检测MAP仍然极具挑战性。最近，有报道称人类内源性逆转录病毒（HERVs）在CD中存在差异基因表达，但关于MAP和HERVs在CD病理中的作用知之甚少。本研究旨在使用间接ELISA法，对来自CD患者以及年龄和性别匹配的健康对照（HCs）的血浆样本中针对HERV-K、HERV-W和MAP抗原的体液免疫反应进行表征。与MAP表位相比，我们观察到CD患者中针对HERV-K和HERV-W表位有显著的抗体反应，并且与HCs相比，患者的总体抗体反应更高。本研究首次报道了CD患者中存在针对HERVs抗原的体液免疫反应。考虑到CD的促炎性质，HERVs可能在遗传易感性个体的疾病发生或发展中起作用。然而，需要进一步研究以更好地理解HERVs在CD中的复杂作用。