Dusza Izabela, Jama Dominika, Skaradziński Grzegorz, Śliwka Paulina, Janek Tomasz, Skaradzińska Aneta
Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, 51-630 Wrocław, Poland.
Molecules. 2025 Apr 15;30(8):1772. doi: 10.3390/molecules30081772.
Biofilms formed by pose therapeutic challenges due to their resistance to conventional antimicrobials, highlighting the need for more effective treatments. Rhamnolipids (RLs) are biosurfactants with diverse antimicrobial properties. Bacteriophages are viruses that target specific bacterial strains. Recent studies have shown that they may affect biofilm formation by fungi and yeasts. This study investigated the combined antimicrobial effects of RLs and bacteriophages against biofilms, focusing on their anti-adhesive and inhibitory effects on biofilm development. RT-PCR assays were used to analyze gene modulation in biofilm formation in response to RLs and bacteriophage treatments, while hyphae formation was examined using microscopy. The results showed that RLs-bacteriophage combinations significantly reduced biofilm formation compared to individual treatments. A combination of 200 mg/L RLs with bacteriophage BF9 led to a 94.8% reduction in biofilm formation. In a subsequent model, the same RL concentration with bacteriophage LO5/1f nearly eliminated biofilm formation (~96%). Gene expression analysis revealed downregulation of key biofilm-associated genes when cells were treated with 200 mg/L RLs and four bacteriophages (BF17, LO5/1f, JG004, FD). These results show the potential of RL and bacteriophage combinations in combating biofilms, presenting a promising therapeutic approach against resilient infections.
由……形成的生物膜由于其对传统抗菌药物具有抗性而带来治疗挑战，这凸显了对更有效治疗方法的需求。鼠李糖脂（RLs）是具有多种抗菌特性的生物表面活性剂。噬菌体是靶向特定细菌菌株的病毒。最近的研究表明它们可能会影响真菌和酵母的生物膜形成。本研究调查了鼠李糖脂和噬菌体对……生物膜的联合抗菌作用，重点关注它们对生物膜形成的抗黏附和抑制作用。使用逆转录聚合酶链反应（RT-PCR）分析来检测在鼠李糖脂和噬菌体处理后……生物膜形成过程中的基因调控，同时使用显微镜检查菌丝形成情况。结果表明，与单独处理相比，鼠李糖脂 - 噬菌体组合显著减少了生物膜的形成。200毫克/升的鼠李糖脂与噬菌体BF9组合使生物膜形成减少了94.8%。在后续模型中，相同浓度的鼠李糖脂与噬菌体LO5/1f几乎消除了生物膜的形成（约96%）。基因表达分析显示，当……细胞用200毫克/升的鼠李糖脂和四种噬菌体（BF17、LO5/1f、JG004、FD）处理时，关键生物膜相关基因表达下调。这些结果显示了鼠李糖脂和噬菌体组合在对抗……生物膜方面的潜力，为应对难治性感染提供了一种有前景的治疗方法。
