基于双柱串联高效液相色谱指纹图谱与超高效液相色谱-四极杆-飞行时间质谱联用的多成分表征与药材质量评价策略
Multi-Component Characterization and Quality Evaluation Strategy of Herba by Combining Dual-Column Tandem HPLC Fingerprint and UPLC-Q-TOF-MS/MS.
作者信息
Zhong Zhijian, Deng Pan, Luo Xiaorong, Zhu Weifeng, Cui Pengdi, Li Zhe, Xiao Zhiqiang, Shen Yu, Wu Xinyu
机构信息
Jiangzhong Pharmaceutical Co., Ltd., Nanchang 330100, China.
Technology and Innovation Center of Jiangxi Traditional Chinese Medicine Manufacturing and Process Quality Control, Nanchang 330004, China.
出版信息
Molecules. 2025 Apr 18;30(8):1825. doi: 10.3390/molecules30081825.
A dual-column tandem mode was used to establish the fingerprints of herb from different origins, and their chemical compositions were characterized by UPLC-Q-TOF-MS/MS, which provided an experimental basis for the establishment of a rapid and efficient method for the overall quality control of herba. For the first time, nine common components were identified from the herba herbs of 24 origins, which were neochlorogenic acid, chlorogenic acid, 4-caffeoylquinic acid, eleutheroside B1, quercetin-3-O-β-D-glucuronide, neoastilbin, astilbin, isofraxidin, and rosmarinic acid, respectively. A total of 92 compounds were identified by liquid mass spectrometry. The quality of the herb from 24 origins was analyzed by similarity evaluation, principal component analysis, and cluster analysis, and the chemical components of herba were identified by UPLC-Q-TOF-MS/MS. The results showed that the overall analysis based on fingerprinting and mass spectrometry could differentiate the origins of the herbs.
采用双柱串联模式建立不同产地药材的指纹图谱，并通过超高效液相色谱-四极杆-飞行时间串联质谱（UPLC-Q-TOF-MS/MS）对其化学成分进行表征，为建立一种快速高效的药材整体质量控制方法提供了实验依据。首次从24个产地的药材中鉴定出9种共有成分，分别为新绿原酸、绿原酸、4-咖啡酰奎宁酸、刺五加苷B1、槲皮素-3-O-β-D-葡萄糖醛酸苷、新橙皮苷二氢查耳酮、橙皮苷二氢查耳酮、异秦皮啶和迷迭香酸。通过液质联用共鉴定出92种化合物。采用相似度评价、主成分分析和聚类分析对24个产地药材的质量进行分析，并通过UPLC-Q-TOF-MS/MS鉴定药材的化学成分。结果表明，基于指纹图谱和质谱的整体分析能够区分药材的产地。
Zotero 插件