两种用于角膜器官培养和消肿的商业培养基的比较：CorneaMax/CorneaJet与Tissue-C/Carry-C。

Comparison of 2 Commercial Media for Corneal Organ Culture and Deswelling: CorneaMax/CorneaJet Versus Tissue-C/Carry-C.

作者信息

Ninotta Sandrine, Toubeau David, Sagnial Tomy, He Zhiguo, Poinard Sylvain, Travers Gauthier, Gain Philippe, Thuret Gilles, Muraine Marc

机构信息

Eye Bank of Saint Etienne, Auvergne Rhône Alpes French Blood Center, Saint-Etienne, France.

Laboratory Biology, Engineering and Imaging for Ophthalmology, Faculty of Medicine, Health Innovation Campus, University Jean Monnet, Saint-Etienne, France.

出版信息

Cornea. 2025 May 6;44(8):1033-1040. doi: 10.1097/ICO.0000000000003876.

DOI:10.1097/ICO.0000000000003876

PMID:40334016

Abstract

PURPOSE

To compare the endothelial cell density (ECD) of corneas stored in organ culture (OC) using CorneaMax/CorneaJet and Tissue-C/Carry-C media.

METHODS

Three complementary experiments included paired corneas stored in CorneaMax or Tissue-C and subsequently deswelled in CorneaJet or Carry-C. In experiment 1, storage media renewal was performed only for CorneaMax as per the manufacturer's guidelines, with storage/deswelling times of 30/4 days (D). In experiment 2, both storage media were renewed on D4, with storage/deswelling time of 28/2 days. In experiment 3, both storage media were renewed, with storage/deswelling times of 22/4 days. In addition to ECD (in the three experiments), the viable ECD (Hoechst 33342/Calcein-AM staining) and central corneal thickness (CCT) were measured in exp. 2.

RESULTS

In experiment 1 (16 pairs), ECD was significantly higher in CorneaMax than in Tissue-C on D30 (2265 ± 379 vs. 2097 ± 344 cells/mm 2 , P = 0.0248) and postdeswelling (2106 ± 405 vs. 1910 ± 355 cells/mm 2 , P = 0.0290). In experiment 2 (10 pairs), ECD did not differ after 28 days of OC; however, CCT was lower on D4, higher on D28 in Tissue-C, and showed no difference after deswelling. Postdeswelling, viable ECD was significantly higher in CorneaJet versus Carry-C (1647 ± 324 vs. 1436 ± 235 cells/mm 2 , P = 0.0189). In experiment 3 (eight pairs), ECD showed no significant difference.

CONCLUSIONS

Endothelial survival is reduced in Tissue-C/Carry-C unless storage is limited to 3 weeks and the medium is renewed. The major corneal edema in Tissue-C may contribute to the reduced ECD.

摘要

目的

比较使用CorneaMax/CorneaJet和Tissue-C/Carry-C培养基在器官培养（OC）中储存的角膜的内皮细胞密度（ECD）。

方法

三项补充实验包括将配对的角膜分别储存在CorneaMax或Tissue-C中，随后在CorneaJet或Carry-C中复水。在实验1中，按照制造商的指南仅对CorneaMax进行储存培养基更换，储存/复水时间为30/4天（D）。在实验2中，两种储存培养基均在第4天更换，储存/复水时间为28/2天。在实验3中，两种储存培养基均进行更换，储存/复水时间为22/4天。除了ECD（在三项实验中），在实验2中还测量了活ECD（Hoechst 33342/钙黄绿素-AM染色）和中央角膜厚度（CCT）。

结果

在实验1（16对）中，在第30天（2265±379对2097±344个细胞/mm²，P = 0.0248）和复水后（2106±405对1910±355个细胞/mm²，P = 0.0290），CorneaMax中的ECD显著高于Tissue-C。在实验2（10对）中，OC 28天后ECD无差异；然而，在第4天CCT较低，在第28天Tissue-C中较高，复水后无差异。复水后，CorneaJet中的活ECD显著高于Carry-C（1647±324对1436±235个细胞/mm²，P = 0.0189）。在实验3（8对）中，ECD无显著差异。