Menaa Bouthaina, Hachicha Rihab, Dubessay Pascal, Abdelkafi Slim, Fendri Imen, Michaud Philippe
Laboratory of Plant Biotechnologies Applied to Crop Improvement, Faculty of Sciences of Sfax, University of Sfax, 3038 Sfax, Tunisia; Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, France.
Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, France.
Biochim Biophys Acta Proteins Proteom. 2025 Jul 1;1873(4):141077. doi: 10.1016/j.bbapap.2025.141077. Epub 2025 May 5.
Some species of microalgae, including Chlorella, can utilize a variety of dissolved organic carbon sources, such as sugars and organic acids, for growth. In the presence of both light and these organic substrates, they exhibit mixotrophic metabolism, combining heterotrophic assimilation of organic carbon with photosynthetic carbon fixation. In this study, we analyzed using proteomic and transcriptomic approaches the ability of Chlorella sp. to shift its metabolism when it was grown in photoautotrophy in BG-11 medium and mixotrophy in 30 % diluted olive mill wastewaters (OMWWs) pretreated with laccases. Using UniProt, InterPro, KEGG Pathway, and Gene Ontology databasesproteomic and transcriptomic data have been analyzed. In mixotrophy, over-expression of cell cycle, signaling, and transport proteins, as well as chaperone proteins, were identified and associated with an overall decrease in photosynthesis and carbohydrate/lipid metabolic pathways. In addition, the expression of light-independent protochlorophyllide reductase, malate synthase, acetyl-CoA carboxylase and pyruvate kinase were modulated. Surprisingly an upregulation of the ammonium transporter protein, which could play a role in OMWWs detoxification was detected. Homology modeling investigation of the three-dimensional structure of ammonium transporter protein revealed that it holds a functional trimeric structure with a lengthy C-terminal region that may be involved in the regulation and activation of ammonium transport.
包括小球藻在内的一些微藻物种能够利用多种溶解有机碳源（如糖类和有机酸）进行生长。在有光和这些有机底物存在的情况下，它们表现出混合营养代谢，即将有机碳的异养同化与光合碳固定相结合。在本研究中，我们使用蛋白质组学和转录组学方法分析了小球藻在BG - 11培养基中进行光自养生长以及在经漆酶预处理的30%稀释橄榄油厂废水（OMWWs）中进行混合营养生长时其代谢转换的能力。利用UniProt、InterPro、KEGG通路和基因本体数据库对蛋白质组学和转录组学数据进行了分析。在混合营养状态下，细胞周期、信号传导、转运蛋白以及伴侣蛋白的过表达被识别出来，并且与光合作用以及碳水化合物/脂质代谢途径的总体下降相关。此外，不依赖光的原叶绿素酸酯还原酶、苹果酸合酶、乙酰辅酶A羧化酶和丙酮酸激酶的表达也受到了调控。令人惊讶的是，检测到铵转运蛋白的上调，其可能在OMWWs解毒中发挥作用。铵转运蛋白三维结构的同源建模研究表明，它具有功能性三聚体结构，带有一个可能参与铵转运调控和激活的长C末端区域。
