Cheng Hang-Yuan, Jiang Li-Ping, Fei Yue, Lu Fei, Ma Shengwei
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, P. R. China.
University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Sci Data. 2025 May 7;12(1):758. doi: 10.1038/s41597-025-05116-3.
Magnaporthe oryzae is a devastating fungal pathogen that causes substantial yield losses in rice and other cereal crops worldwide. A high-quality genome assembly is critical for addressing challenges posed by this pathogen. However, the current widely used MG8 assembly of the M. oryzae strain 70-15 reference genome contains numerous gaps and unresolved repetitive regions. Here, we report a complete 44.82 Mb high-quality nuclear genome and a 35.95 kb circular mitochondrial genome for strain 70-15, generated using deep-coverage PacBio high-fidelity sequencing (HiFi) and high-resolution chromatin conformation capture (Hi-C) data. Notably, we successfully resolved one or both telomere sequences for all seven chromosomes and achieved telomere-to-telomere (T2T) assemblies for chromosomes 2, 3, 4, 6, and 7. Based on this T2T assembly, we predicted 12,100 protein-coding genes and 493 effectors. This high-quality T2T assembly represents a significant advancement in M. oryzae genomics and provides an enhanced reference for studies in genome biology, comparative genomics, and population genetics of this economically important plant pathogen.
稻瘟病菌是一种具有毁灭性的真菌病原体，在全球范围内导致水稻和其他谷类作物大幅减产。高质量的基因组组装对于应对这种病原体带来的挑战至关重要。然而，目前广泛使用的稻瘟病菌70-15参考基因组的MG8组装版本包含许多缺口和未解决的重复区域。在此，我们报告了70-15菌株完整的44.82兆碱基高质量核基因组和35.95千碱基的环状线粒体基因组，这些是利用深度覆盖的PacBio高保真测序（HiFi）和高分辨率染色质构象捕获（Hi-C）数据生成的。值得注意的是，我们成功解析了所有七条染色体的一个或两个端粒序列，并实现了第2、3、4、6和7号染色体的端粒到端粒（T2T）组装。基于此T2T组装，我们预测了12100个蛋白质编码基因和493个效应子。这种高质量的T2T组装代表了稻瘟病菌基因组学的重大进展，并为这种经济上重要的植物病原体的基因组生物学、比较基因组学和群体遗传学研究提供了一个更好的参考。
