Deepali Deepali, Tejoprakash N, Sudhakara Reddy M
Department of Energy and Environment, Thapar Institute of Engineering & Technology, Patiala, Punjab, 147004, India.
Department of Biotechnology, Thapar Institute of Engineering & Technology, Patiala, Punjab, 147004, India.
Curr Microbiol. 2025 May 7;82(6):285. doi: 10.1007/s00284-025-04245-7.
The present study aimed to investigate the diversity of bacterial communities in seleniferous soils using Illumina Mi-Seq Next-Generation Sequencing. This study also compared seleniferous soils (SE) with non-seleniferous (NS) soils to evaluate Selenium (Se) impact on microbial communities and soil properties. Metagenomic analysis identified Proteobacteria as the predominant phylum in both environments, with SE soils exhibiting a higher dominance (48%) than NS soils (31%). The most dominant operational taxonomic unit (OTU) across both soil types belonged to the genus Bacillus. Se altered microbial community composition, increasing the abundance of the Bacillaceae family (30%) and Pseudomonadaceae family (25%) compared to NS soil. Bacillus was the dominant genus in the SE environment indicating its tolerance to selenium. Diversity indices indicated that control soils had higher species richness, while SE soils exhibited a more stressed microbial structure. A consortium of bacterial isolates (Proteus terrae Se3, Halopseudomonas formosensis Se5, and Corynebacterium glutamicum Se38) was inoculated in maize plants cultivated in natural seleniferous soils. Plants inoculated with bacterial consortium grew more healthy and had greater biomass in their roots, shoots, and seeds. Bacterial inoculation results in lesser selenium accumulation in the roots, shoots and seeds of maize plants compared to non-inoculated plants. These results suggest that bacterial species from seleniferous soils may be employed as biofertilizers to enhance plant growth and help plants tolerate Se toxicity in seleniferous soils.
本研究旨在利用Illumina Mi-Seq下一代测序技术调查富硒土壤中细菌群落的多样性。本研究还将富硒土壤（SE）与非富硒土壤（NS）进行比较，以评估硒（Se）对微生物群落和土壤性质的影响。宏基因组分析确定变形菌门是两种环境中的主要门类，SE土壤中的优势度（48%）高于NS土壤（31%）。两种土壤类型中最主要的可操作分类单元（OTU）属于芽孢杆菌属。与NS土壤相比，硒改变了微生物群落组成，增加了芽孢杆菌科（30%）和假单胞菌科（25%）的丰度。芽孢杆菌是SE环境中的优势属，表明其对硒具有耐受性。多样性指数表明对照土壤的物种丰富度更高，而SE土壤的微生物结构压力更大。将一组细菌分离株（土变形杆菌Se3、台湾盐假单胞菌Se5和谷氨酸棒状杆菌Se38）接种到在天然富硒土壤中种植的玉米植株上。接种细菌联合体的植株生长更健康，根、茎和种子的生物量更大。与未接种的植株相比，细菌接种导致玉米植株根、茎和种子中的硒积累减少。这些结果表明，来自富硒土壤的细菌物种可作为生物肥料用于促进植物生长，并帮助植物耐受富硒土壤中的硒毒性。
