Dorokhov Iu L, Aleksandrova N M, Atabekov I G
Mol Biol (Mosk). 1985 May-Jun;19(3):654-61.
The possibility of infection of tobacco upper leaves with tobacco mosaic virus (TMV) was examined in experiments where the inoculum was imbibed through the cut stem. The inoculum used were: a) a preparation of a virus-specific informosome-like ribonucleoproteins (vRNP) isolated from TMV-infected plants; b) a TMV preparation; or c) a mixture of TMV and vRNP. Multiplication of TMV in upper leaves was observed in neither of the variants; nevertheless in the vascular tissue and/or probably in adjoining parenchymal cells, two kinds of RNA were synthesized: of mol. w. (1.1--1.3) X 10(6) and (0.6--0.8) X 10(6). These RNA were not found in healthy plants in the presence of actinomycin D. The synthesis of genomic TMV RNA is suppressed under these conditions. Thus, some kind of abortive TMV infection takes place under the condition of experimental inoculation of plants through a cut stem. Molecular hybridization with the DNA of recombinant plasmid containing a nucleotide sequence complementary to the 3'-portion of genomic TMV RNA proves that short RNAs synthesized under the abortive infection conditions are TMV-specific. The experiments with differential temperature treatment of N-gene-containing plants under abortive infection conditions suggest that necrotization is not necessarily induced by genomic TMV RNA synthesis.
在通过切割茎部吸收接种物的实验中，研究了烟草上部叶片感染烟草花叶病毒（TMV）的可能性。所用的接种物有：a）从感染TMV的植物中分离出的病毒特异性信息体样核糖核蛋白（vRNP）制剂；b）TMV制剂；或c）TMV与vRNP的混合物。在任何一种变体中均未观察到TMV在上部叶片中的增殖；然而，在维管组织和/或可能在相邻的薄壁细胞中，合成了两种RNA：分子量为（1.1 - 1.3）×10⁶和（0.6 - 0.8）×10⁶。在放线菌素D存在的情况下，健康植物中未发现这些RNA。在这些条件下，基因组TMV RNA的合成受到抑制。因此，在通过切割茎部对植物进行实验接种的条件下，发生了某种流产型TMV感染。与含有与基因组TMV RNA的3'部分互补的核苷酸序列的重组质粒DNA进行分子杂交证明，在流产感染条件下合成的短RNA是TMV特异性的。在流产感染条件下对含N基因的植物进行变温处理的实验表明，坏死不一定是由基因组TMV RNA合成诱导的。
