Dorokhov Iu L, Miroshnichenko N A, Aleksandrova N M, Atabekov I G
Mol Biol (Mosk). 1984 Jul-Aug;18(4):1001-10.
Informosome-like virus-specific ribonucleoprotein (vRNP) of tobacco mosaic virus (TMV) comprise a set of four major polypeptides having molecular weights of 17 500, 31 000, 37 000 and 39 000. Of the minor polypeptides, those of apparent molecular weights 25 000, 55 000, 68 000 and 70 000 had electrophoretic mobilities of polypeptides found in a ribonucleoprotein preparation from uninoculated plants. Polypeptide with mol.wt. 175 000 is TMV coat protein so far as: a) vRNP was precipitated with immunoglobulins against TMV and TMV coat protein; b) it had electrophoretic mobility similar to mobility of TMV coat protein; c) the peptide map of polypeptides with mol.wts 31 000, 37 000 and 39 000 are probably virus-specific-products. This is supposed because they are not present in cell informosomes protein, and they are not revealed in vRNP induced in cells after infection with potato virus X (PVX). Electrophoresis of vRNP-PVX protein reveals polypeptides of 23 000 (PVX coat protein), 55 000, 70 000, 78 000, 95 000, 120 000 and 145 000.
烟草花叶病毒（TMV）的类信息体病毒特异性核糖核蛋白（vRNP）由一组四种主要多肽组成，其分子量分别为17500、31000、37000和39000。在次要多肽中，表观分子量为25000、55000、68000和70000的多肽的电泳迁移率与从未接种植物的核糖核蛋白制剂中发现的多肽的电泳迁移率相同。分子量为175000的多肽是TMV外壳蛋白，依据如下：a）vRNP可被抗TMV和TMV外壳蛋白的免疫球蛋白沉淀；b）其电泳迁移率与TMV外壳蛋白的迁移率相似；c）分子量为31000、37000和39000的多肽的肽图谱可能是病毒特异性产物。之所以这样推测，是因为它们不存在于细胞信息体蛋白中，并且在用马铃薯X病毒（PVX）感染后细胞中诱导产生的vRNP中也未显现。vRNP - PVX蛋白的电泳显示出分子量为23000（PVX外壳蛋白）、55000、70000、78000、95000、120000和145000的多肽。
