通过DNA转移重建活性表面T3/T细胞抗原受体。
Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer.
作者信息
Ohashi P S, Mak T W, Van den Elsen P, Yanagi Y, Yoshikai Y, Calman A F, Terhorst C, Stobo J D, Weiss A
出版信息
Nature. 1985;316(6029):606-9. doi: 10.1038/316606a0.
DOI:10.1038/316606a0
PMID:4033759
Abstract
We have introduced a full-length complementary DNA of the T-cell antigen receptor beta-chain into a mutant human T-cell line that lacked a complete beta-chain messenger RNA, had a diminished level of alpha-chain transcript and did not express surface T3- or antigen receptor. Expression of the transfected beta-chain led to a normal level of alpha-chain transcript and a structurally and functionally active T3 T-cell antigen receptor complex on the cell surface.
摘要
我们已将T细胞抗原受体β链的全长互补DNA导入一个突变的人T细胞系，该细胞系缺乏完整的β链信使RNA，α链转录物水平降低，且不表达表面T3或抗原受体。转染的β链的表达导致α链转录物达到正常水平，并在细胞表面形成结构和功能均活跃的T3 T细胞抗原受体复合物。
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