Taghavi Mahnour Alsadat, Ahmadi Maryam, Dehghan-Nayeri Davoud, Salehi Zahra, Shams-Ghahfarokhi Masoomeh, Jamzivar Fatemehsadat, Razzaghi-Abyaneh Mehdi
Department of Microbiology, Faculty of Pharmacy, Islamic Azad University, Tehran, Iran.
Department of Mycology, Pasteur Institute of Iran, Tehran, Iran.
Iran J Microbiol. 2025 Apr;17(2):303-311. doi: 10.18502/ijm.v17i2.18397.
The increasing prevalence of fungal infections due to antifungal resistance underscores the need for novel treatment strategies. The present study aimed to investigate the inhibitory effects of soil-originated antagonistic bacteria against and species.
Fifty soil samples collected from Isfahan and Khuzestan provinces by using the Zig-Zag method were cultured on glucose-yeast extract (GY) agar around fungal colonies to isolate antagonistic bacteria. Antifungal activity was assessed by measuring clear zones around the colonies of and by co-culture linear method. Potent antagonistic bacteria were identified by 16S rRNA sequencing, and evaluated for antifungal activity using disk diffusion assays compared with amphotericin B and ketoconazole.
Among 50 samples, fifteen showed antifungal effects, yielding 55 bacterial strains. Four isolates with strong antifungal activity against all tested fungi were identified as and sp. These bacteria were distributed in distinct clusters phylogenitically and showed diverse antifungal activity.
The results suggest the potential of soil-derived species as promising antifungal agents. Further studies are recommended to identify their inhibitory metabolites, their ability as biocontrol agents against soil habitated fungi and to explore their mechanism of action and spectrum of activity.
由于抗真菌耐药性导致真菌感染的患病率不断上升，凸显了新型治疗策略的必要性。本研究旨在调查土壤来源的拮抗细菌对[具体真菌1]和[具体真菌2]物种的抑制作用。
采用Zig-Zag方法从伊斯法罕省和胡齐斯坦省采集的50份土壤样本，在真菌菌落周围的葡萄糖酵母提取物（GY）琼脂上培养，以分离拮抗细菌。通过共培养线性方法测量[具体真菌1]和[具体真菌2]菌落周围的透明圈来评估抗真菌活性。通过16S rRNA测序鉴定强效拮抗细菌，并与两性霉素B和酮康唑相比，使用纸片扩散法评估其抗真菌活性。
在50个样本中，15个显示出抗真菌作用，产生了55株细菌菌株。对所有测试真菌具有强抗真菌活性的4株分离株被鉴定为[具体细菌1]和[具体细菌2]种。这些细菌在系统发育上分布在不同的簇中，并表现出不同的抗真菌活性。
结果表明土壤来源的[具体细菌]物种作为有前景的抗真菌剂的潜力。建议进一步研究以确定其抑制性代谢产物、作为针对土壤栖息真菌的生物防治剂的能力，并探索其作用机制和活性谱。
