Tao Weijian, Mihoubi Fatma, Patrahau Bianca, Bonfio Claudia, Nordén Bengt, Ebbesen Thomas W
ISIS & icFRC, University of Strasbourg & CNRS, Strasbourg, France.
Department of Biochemistry, University of Cambridge, Cambridge, UK.
QRB Discov. 2025 Mar 10;6:e13. doi: 10.1017/qrd.2025.5. eCollection 2025.
Manipulating matter by strong coupling to the vacuum field has attracted intensive interests over the last decade. In particular, vibrational strong coupling (VSC) has shown great potential for modifying ground state properties in solution chemistry and biochemical processes. In this work, the effect of VSC of water on the melting behaviour of ds-DNA, an important biophysical process, is explored. Several experimental conditions, including the concentration of ds-DNA, cavity profile, solution environment, as well as thermal annealing treatment, were tested. No significant effect of VSC was observed for the melting behaviour of the ds-DNA sequence used. This demonstrates yet again the robustness of ds-DNA to outside perturbations. Our work also provides a general protocol to probe the effects of VSC on biological systems inside microfluid Fabry-Perot cavities and should be beneficial to better understand and harness this phenomenon.
在过去十年中，通过与真空场强耦合来操纵物质引起了广泛关注。特别是，振动强耦合（VSC）在改变溶液化学和生化过程中的基态性质方面显示出巨大潜力。在这项工作中，探索了水的VSC对双链DNA（ds-DNA）熔化行为（一个重要的生物物理过程）的影响。测试了几个实验条件，包括ds-DNA的浓度、腔轮廓、溶液环境以及热退火处理。对于所使用的ds-DNA序列的熔化行为，未观察到VSC的显著影响。这再次证明了ds-DNA对外部扰动的稳健性。我们的工作还提供了一个通用方案，用于探测微流体法布里-珀罗腔内VSC对生物系统的影响，并且应该有助于更好地理解和利用这一现象。
