Keith C H, Ratan R, Maxfield F R, Bajer A, Shelanski M L
Nature. 1985;316(6031):848-50. doi: 10.1038/316848a0.
Cytoplasmic free calcium has been proposed as a regulator of many microtubule-mediated processes, including mitosis. It has been difficult to test this hypothesis because methods for local measurement of free Ca2+ in the living cell have not been available. We have used the fluorescent calcium indicator dye Quin-2 (methoxyquinoline-1bis(o-aminophenoxy)ethane-N,N,N',N' -tetra acetic acid), which allows such observations to be made by digital processing of fluorescent images from the light microscope. Here we report the application of this technique to the study of local Ca2+ concentrations in mitotic endosperm cells of Haemanthus sp., and show that there is transient increase in free Ca2+ at the mitotic spindle poles during anaphase. This locally high Ca2+ may provide a mechanism for the regional control of microtubules and other cytoskeletal elements during anaphase.
细胞质游离钙已被认为是许多微管介导过程（包括有丝分裂）的调节因子。由于一直没有在活细胞中局部测量游离Ca2+的方法，因此难以验证这一假设。我们使用了荧光钙指示剂染料喹啉-2（甲氧基喹啉-1-双（邻氨基苯氧基）乙烷-N，N，N'，N'-四乙酸），通过对来自光学显微镜的荧光图像进行数字处理来进行此类观察。在此，我们报告了该技术在研究海葱属有丝分裂胚乳细胞中局部Ca2+浓度方面的应用，并表明在后期有丝分裂纺锤体极处游离Ca2+有短暂增加。这种局部高Ca2+可能为后期微管和其他细胞骨架成分的区域控制提供一种机制。
