Ju Jiaming, Lu Xinhe, Gao Ziqing, Yin Hongyan, Xu Shunqing, Li Hanzeng
School of Life and Health Sciences, Hainan University, Haikou 570228, China.
School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
Int J Mol Sci. 2025 Apr 13;26(8):3673. doi: 10.3390/ijms26083673.
To date, species have been identified from diverse sources with various ecological and functional roles. However, the genomic features and pathogenic potential of antibiotic-resistant strains remain understudied. Here, we isolated the sp. HNU-1 from tropical Hainan Province, China, and found it can induce diapause in following ingestion, while exhibiting no significant effects on the nematode's lifespan, survival rate, locomotion, and intestinal epithelial cells. This bacterium demonstrates resistance to multiple antibiotics, including kanamycin, streptomycin, sulfonamides, and vancomycin. On LB medium, sp. HNU-1 forms yellow, opaque colonies with a smooth, moist surface, regular edges, a convex center, and no surrounding halo, with diameters ranging from 2 to 3 mm. Furthermore, we performed whole-genome sequencing using third-generation high-throughput sequencing technology. De novo assembly revealed a genome size of 3,375,033 bp, with a GC content of 70.37%. A total of 3270 functional genes, accounting for 88.98% of the genome, were annotated, along with six potential CRISPR sequences and other genetic elements. Genomic and bioinformatic analyses further identified antibiotics-related genes. This research provides a theoretical foundation for investigating antibiotic-resistant environmental bacteria in tropical environments and offers new insights into potential therapeutic strategies for microbial infections and host-microbe interactions.
迄今为止，已从具有各种生态和功能作用的不同来源中鉴定出多种物种。然而，对抗生素耐药菌株的基因组特征和致病潜力仍研究不足。在此，我们从中国热带海南省分离出了嗜线虫致病杆菌属（Photorhabdus）HNU - 1菌株，发现其在被线虫摄入后可诱导线虫进入滞育状态，同时对该线虫的寿命、存活率、运动能力和肠道上皮细胞无显著影响。这种细菌对多种抗生素具有抗性，包括卡那霉素、链霉素、磺胺类药物和万古霉素。在LB培养基上，嗜线虫致病杆菌属HNU - 1菌株形成黄色、不透明的菌落，表面光滑、湿润，边缘规则，中心凸起，周围无晕圈，直径范围为2至3毫米。此外，我们使用第三代高通量测序技术进行了全基因组测序。从头组装显示基因组大小为3,375,033 bp，GC含量为70.37%。共注释了3270个功能基因，占基因组的88.98%，同时还发现了六个潜在的CRISPR序列和其他遗传元件。基因组和生物信息学分析进一步鉴定出了与抗生素相关的基因。本研究为调查热带环境中的抗生素耐药环境细菌提供了理论基础，并为微生物感染的潜在治疗策略和宿主 - 微生物相互作用提供了新的见解。
