Hashimoto Mina, Tanimoto Yoshihiko, Kage-Nakadai Eriko
Department of Nutrition, Graduate School of Human Life and Ecology, Osaka Metropolitan University, Osaka, Japan.
Institute for Life and Medical Sciences, Kyoto University, Kyoto, Japan.
Biosci Biotechnol Biochem. 2025 Jul 23;89(8):1236-1247. doi: 10.1093/bbb/zbaf065.
Lactiplantibacillus brownii was first discovered in 2023. We previously isolated L. brownii strain MH-1 from homemade pickled Chinese cabbage in Hiroshima Prefecture, Japan, and performed whole-genome sequencing. In this study, we examined the beneficial effects of L. brownii strain MH-1 on a host using C. elegans. MH-1 and heat-killed MH-1 extended the lifespan and also alleviated motility decline during aging in worms. Feeding MH-1 to nematodes indicated its presence in the intestinal tract with no change in body or brood sizes, suggesting that dietary restrictions did not occur. There were no significant differences in intestinal barrier enhancement or fat accumulation in MH-1-fed C. elegans. The stress response of C. elegans was enhanced by MH-1 for oxidative stress tolerance. MH-1 increased the expression of genes involved in defense response and lipid catabolism. These results demonstrate the beneficial effects of MH-1 on nematodes and its potential use as a probiotic.
布氏乳酸杆菌于2023年首次被发现。我们之前从日本广岛县的自制泡菜中分离出布氏乳酸杆菌MH-1菌株，并进行了全基因组测序。在本研究中，我们使用秀丽隐杆线虫研究了布氏乳酸杆菌MH-1菌株对宿主的有益作用。MH-1和热灭活的MH-1延长了线虫的寿命，并缓解了其衰老过程中的运动能力下降。给线虫喂食MH-1表明其存在于肠道中，且虫体大小和产卵量没有变化，这表明没有发生饮食限制。喂食MH-1的秀丽隐杆线虫在肠道屏障增强或脂肪积累方面没有显著差异。MH-1增强了秀丽隐杆线虫对氧化应激的耐受能力的应激反应。MH-1增加了参与防御反应和脂质分解代谢的基因的表达。这些结果证明了MH-1对线虫的有益作用及其作为益生菌的潜在用途。
