Yang Benshun, Wang Meng, Wu Zhangqing, Tan Jianbing, Meng Yanhong, Zhang Taoping, Zan Linsen, Yang Wucai
College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Shaanxi Qinbao Animal Husbandry Co., LTD, Yangling, China.
Anim Biotechnol. 2025 Dec;36(1):2497915. doi: 10.1080/10495398.2025.2497915. Epub 2025 May 8.
This study investigates the role of Perilipin1 (PLIN1) in milk fat synthesis in bovine mammary epithelial cells (BMECs) and its regulatory mechanisms, aiming to provide a foundation for improving milk fat content through molecular breeding. BMECs were used as a model to analyze the effects of PLIN1 overexpression (OE-PLIN1) and interference (si-PLIN1) on milk fat synthesis and lipid-related gene expression using RT-qPCR, Western blot, and Oil Red O staining. Results show that OE-PLIN1 significantly enhances triglyceride (TAG) accumulation in BMECs (P < 0.01), upregulates lipid synthesis-related genes (such as PPARγ, C/EBPα, C/EBPβ, FABP4, FASN) (P < 0.05), and downregulates the mRNA expression of lipid breakdown-related genes (HSL, ATGL) (P < 0.05). Conversely, si-PLIN1 significantly reduces TAG accumulation (P < 0.05) and lowers the expression of lipid synthesis and breakdown genes (P < 0.05). Additionally, OE-PLIN1 combined with SREBP1 siRNA interference (si-SREBP1) did not have a significant impact on the mRNA and protein levels of SREBP1, but it significantly altered SREBP1's phosphorylation, indicating that SREBP1 interference inhibits PLIN1's effect on milk fat synthesis. This study suggests that PLIN1 promotes milk fat synthesis in BMECs via regulating SREBP1 activity, offering a new strategy for enhancing milk fat content in dairy cattle.
本研究调查了 perilipin1（PLIN1）在牛乳腺上皮细胞（BMECs）乳脂肪合成中的作用及其调控机制，旨在为通过分子育种提高乳脂肪含量提供基础。以 BMECs 为模型，采用 RT-qPCR、蛋白质免疫印迹和油红 O 染色分析 PLIN1 过表达（OE-PLIN1）和干扰（si-PLIN1）对乳脂肪合成及脂质相关基因表达的影响。结果显示，OE-PLIN1 显著增强了 BMECs 中甘油三酯（TAG）的积累（P < 0.01），上调了脂质合成相关基因（如 PPARγ、C/EBPα、C/EBPβ、FABP4、FASN）的表达（P < 0.05），并下调了脂质分解相关基因（HSL、ATGL）的 mRNA 表达（P < 0.05）。相反，si-PLIN1 显著降低了 TAG 的积累（P < 0.05），并降低了脂质合成和分解基因的表达（P < 0.05）。此外，OE-PLIN1 与 SREBP1 小干扰 RNA（si-SREBP1）联合使用对 SREBP1 的 mRNA 和蛋白水平没有显著影响，但显著改变了 SREBP1 的磷酸化，表明 SREBP1 干扰抑制了 PLIN1 对乳脂肪合成的作用。本研究表明，PLIN1 通过调节 SREBP1 的活性促进 BMECs 中的乳脂肪合成，为提高奶牛乳脂肪含量提供了一种新策略。
