Liu Yamei, Wang Chaohong, Ouyang Lejun, Li Limei, Su Min
College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, China.
College of Life and Geographic Sciences, The Key Laboratory of Ecology and Biological Resources in Yarkand Oasis at Colleges and Universities under the Department of Education of Xinjiang Uygur Autonomous Region, Kashi University, Kashi, China.
PLoS One. 2025 May 8;20(5):e0322224. doi: 10.1371/journal.pone.0322224. eCollection 2025.
Eucalyptus urophylla X Eucalyptus grandis is an important afforestation hybrid clone that supports wood safety in China. To explore the mechanism of callus formation and differentiation of different types of E. urophylla × E. grandis, we cultivated four different types of E. urophylla × E. grandis calli, measured their enzyme activities and endogenous hormones, and sequenced them at the transcriptome level. Transcriptome analysis revealed that there were significant differences in the clustering of differentially expressed genes. Compared with the green calli, 2203, 2485, and 2078 differentially expressed genes were identified in the red, white, and yellow calli, respectively. Differentially expressed genes were involved in metabolic processes, biological regulation, signal transduction, stimulus response, catalytic activity, and binding, such as GRFs gene. Combined with the changes in physiological indices and transcription levels, we revealed the regulatory characteristics of substance storage and antioxidant capacity in the process of callus differentiation of E. urophylla × E. grandis, which contributes to the understanding of the mechanism of plant cell growth and differentiation.
尾叶桉×巨桉是中国保障木材安全的重要造林杂交无性系。为探究不同类型尾叶桉×巨桉愈伤组织形成与分化的机制，我们培养了四种不同类型的尾叶桉×巨桉愈伤组织，测定其酶活性和内源激素，并在转录组水平进行测序。转录组分析表明，差异表达基因的聚类存在显著差异。与绿色愈伤组织相比，在红色、白色和黄色愈伤组织中分别鉴定出2203、2485和2078个差异表达基因。差异表达基因参与代谢过程、生物调控、信号转导、刺激反应、催化活性和结合等，如GRFs基因。结合生理指标和转录水平的变化，揭示了尾叶桉×巨桉愈伤组织分化过程中物质储存和抗氧化能力的调控特征，有助于理解植物细胞生长和分化的机制。
