Key Laboratory of State Forestry and Grassland Administration on Tropical Forestry, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China.
Xinhui Research Institute of Forestry Science, Jiangmen 529100, China.
Genes (Basel). 2024 Jul 17;15(7):930. doi: 10.3390/genes15070930.
is an important planted hardwood tree worldwide with fast growth and good wood performance. The nitrate transporter (NRT) gene family is a major core involved in nitrogen (N) absorption and utilization in plants, but the comprehensive characterization of genes in remains mostly elusive. In this study, a total of 75 genes were identified from the genome of that were distributed unevenly across ten chromosomes, except Chr9. A phylogenetic analysis showed that the EgNRT proteins could be divided into three classes, namely NRT1, NRT2 and NRT3, which contained 69, 4 and 2 members, respectively. The -regulatory elements in the promoter regions of genes were mainly involved in phytohormone and stress response. The transcriptome analysis indicated that the differentially expressed genes of leaf and root in under different N supply conditions were mainly involved in the metabolic process and plant hormone signal transduction. In addition, the transcriptome-based and RT-qPCR analysis revealed that the expression of 13 genes, especially , , and , was significantly upregulated in the root under low-N-supply treatment, suggesting that those genes might play a critical role in root response to nitrate deficiency. Taken together, these results would provide valuable information for characterizing the roles of EgNRTs and facilitate the clarification of the molecular mechanism underlying -mediated N absorption and distribution in .
是一种重要的人工种植硬木树种，具有生长迅速和木材性能良好的特点。硝酸盐转运蛋白（NRT）基因家族是植物氮（N）吸收和利用的主要核心，但是在中基因的综合特征在很大程度上仍难以捉摸。在这项研究中，从的基因组中鉴定出了总共 75 个基因，这些基因不均匀地分布在 10 条染色体上，除了 Chr9 外。系统发育分析表明，EgNRT 蛋白可分为 3 类，即 NRT1、NRT2 和 NRT3，分别包含 69、4 和 2 个成员。基因启动子区的-调节元件主要参与植物激素和应激反应。转录组分析表明，在不同氮供应条件下叶和根的差异表达基因主要参与代谢过程和植物激素信号转导。此外，基于转录组和 RT-qPCR 的分析表明，13 个基因的表达，特别是 、 、 、 ，在低氮供应处理下的根中显著上调，表明这些基因可能在根对硝酸盐缺乏的反应中发挥关键作用。总之，这些结果将为描述 EgNRTs 的作用提供有价值的信息，并有助于阐明介导的 N 吸收和分布的分子机制。
