Lagunas-Cortés Noé, García-Sánchez Brenda Yomara, Ríos-Castro Emmanuel, Vega-López Marco Antonio, González-Pozos Sirenia, Diaz-Martín Rubén Darío, Carranza Juan Manuel, Ramírez-Flores Carlos J, Mondragón-Flores Ricardo
Departamento de Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Av. IPN No. 2508, Ciudad de Mexico, 07360, Mexico.
Unidad de Genómica, Proteómica y Metabolómica, LaNSE, Cinvestav, Mexico.
J Proteomics. 2025 Jun 15;317:105454. doi: 10.1016/j.jprot.2025.105454. Epub 2025 May 6.
Research on the immunogenic molecules of Toxoplasma is a key priority in the development of protective vaccines against the parasite. In the present study, we analyzed the profile of immunorecognized proteins from the Toxoplasma cytoskeleton using sera from patients with both acute and chronic toxoplasmosis. The immunorecognized spots were analyzed by mass spectrometry and characterized by bioinformatic methods, leading to the identification of a total of 313 proteins. Sixty-three antigenic proteins were recognized by IgM antibodies and 250 antigenic proteins were recognized by IgG antibodies. About 10 proteins specifically reported as cytoskeletal proteins were identified with the IgG antibodies while 9 cytoskeletal proteins were detected by IgM antibodies. Bioinformatic analyses of the identified antigenic proteins were performed to determine their immunogenic potential, including the number of epitopes recognized by B lymphocytes, cytotoxic T lymphocytes (CD8+), and helper T lymphocytes (CD4+) receptors. This analysis enabled the selection of highly immunogenic proteins, which could serve as potential candidates for the design of a future vaccine against toxoplasmosis. SIGNIFICANCE: The study of immunogenic molecules from Toxoplasma gondii is a key priority in the search for protective vaccines. Despite partial success in previous strategies, identifying immunogenic proteins from the T. gondii cytoskeleton using immune-proteomics and bioinformatic approaches is crucial for vaccine development. This study provides valuable data that could serve as the foundation for designing novel immunogenic and immunoprotective molecules against toxoplasmosis.
弓形虫免疫原性分子的研究是开发针对该寄生虫的保护性疫苗的关键优先事项。在本研究中，我们使用急性和慢性弓形虫病患者的血清分析了弓形虫细胞骨架的免疫识别蛋白谱。通过质谱分析免疫识别的斑点并用生物信息学方法进行表征，共鉴定出313种蛋白质。63种抗原蛋白被IgM抗体识别，250种抗原蛋白被IgG抗体识别。IgG抗体鉴定出约10种特别报道为细胞骨架蛋白的蛋白质，而IgM抗体检测到9种细胞骨架蛋白。对鉴定出的抗原蛋白进行生物信息学分析以确定其免疫原性潜力，包括B淋巴细胞、细胞毒性T淋巴细胞（CD8 +）和辅助性T淋巴细胞（CD4 +）受体识别的表位数量。该分析有助于选择高度免疫原性的蛋白质，这些蛋白质可作为未来抗弓形虫病疫苗设计的潜在候选物。意义：研究刚地弓形虫的免疫原性分子是寻找保护性疫苗的关键优先事项。尽管先前的策略取得了部分成功，但使用免疫蛋白质组学和生物信息学方法从弓形虫细胞骨架中鉴定免疫原性蛋白质对于疫苗开发至关重要。本研究提供了有价值的数据，可为设计抗弓形虫病的新型免疫原性和免疫保护性分子奠定基础。
