Wang Xiao-Dong, Ma Bao-Ying, Lai Shi-Ying, Cai Xiang-Jing, Cong Yan-Guang, Xu Jun-Fa, Zhang Peng-Fei
Dongguan Key Laboratory of Pathogenesis and Experimental Diagnosis of Infectious Diseases, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China.
Institute of Laboratory Medicine, School of Medical Technology, Guangdong Medical University, Dongguan, China.
J Biol Eng. 2025 May 8;19(1):41. doi: 10.1186/s13036-025-00513-z.
Antibodies characterized by high affinity and specificity, developed through high-throughput screening and rapid preparation, are crucial to contemporary biomedical industry. Traditional antibody preparation via the hybridoma strategy faces challenges like low efficiency, long manufacturing cycles, batch variability and labor intensity. Advances in molecular biology and gene editing technologies offer revolutionary improvements in antibody production. New high-throughput technologies like antibody library display, single B cell antibody technologies, and single-cell sequencing have significantly cut costs and boosted the efficiency of antibody development. These innovations accelerate commercial applications of antibodies, meeting the biopharmaceutical industry's evolving demands. This review explores recent advancements in high-throughput development of antibody, highlighting their potential advantages over traditional methods and their promising future.
通过高通量筛选和快速制备得到的具有高亲和力和特异性的抗体，对当代生物医学产业至关重要。传统的通过杂交瘤策略制备抗体面临着效率低、生产周期长、批次差异和劳动强度大等挑战。分子生物学和基因编辑技术的进步为抗体生产带来了革命性的改进。抗体文库展示、单细胞B抗体技术和单细胞测序等高通量新技术显著降低了成本，提高了抗体开发效率。这些创新加速了抗体的商业应用，满足了生物制药行业不断变化的需求。本文综述探讨了抗体高通量开发的最新进展，强调了它们相对于传统方法的潜在优势及其光明的未来。
