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特异性单克隆兔抗体分离技术的进展

Advances in the Isolation of Specific Monoclonal Rabbit Antibodies.

作者信息

Zhang Zaibao, Liu Huijuan, Guan Qian, Wang Lei, Yuan Hongyu

机构信息

Institute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China.

College of Life Science, Xinyang Normal University, Xinyang, China.

出版信息

Front Immunol. 2017 May 5;8:494. doi: 10.3389/fimmu.2017.00494. eCollection 2017.

DOI:10.3389/fimmu.2017.00494
PMID:28529510
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5418221/
Abstract

The rabbit monoclonal antibodies (mAbs) have advantages in pharmaceuticals and diagnostics with high affinity and specificity. During the past decade, many techniques have been developed for isolating rabbit mAbs, including single B cell antibody technologies. This review describes the basic characterization of rabbit antibody repertoire and summarizes methods of hybridoma technologies, phage display platform, and single B cell antibody technologies. With advances in antibody function and repertoire analysis, rabbit mAbs will be widely used in therapeutic applications in the coming years.

摘要

兔单克隆抗体（mAbs）在制药和诊断领域具有优势，具有高亲和力和特异性。在过去十年中，已经开发了许多用于分离兔单克隆抗体的技术，包括单B细胞抗体技术。本综述描述了兔抗体库的基本特征，并总结了杂交瘤技术、噬菌体展示平台和单B细胞抗体技术的方法。随着抗体功能和库分析的进展，兔单克隆抗体在未来几年将广泛应用于治疗领域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56e9/5418221/bf1816fa386d/fimmu-08-00494-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56e9/5418221/bf1816fa386d/fimmu-08-00494-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56e9/5418221/bf1816fa386d/fimmu-08-00494-g001.jpg

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3
Generation of Recombinant Monoclonal Antibodies from Immunised Mice and Rabbits via Flow Cytometry and Sorting of Antigen-Specific IgG+ Memory B Cells.通过流式细胞术和抗原特异性IgG⁺记忆B细胞分选从免疫小鼠和兔中产生重组单克隆抗体
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Pept Sci (Hoboken). 2024 Mar;116(2). doi: 10.1002/pep2.24333. Epub 2023 Sep 18.
4
Anti-Immune Complex Antibodies are Elicited During Repeated Immunization with HIV Env Immunogens.在使用HIV包膜免疫原进行重复免疫期间会引发抗免疫复合物抗体。
bioRxiv. 2024 Mar 17:2024.03.15.585257. doi: 10.1101/2024.03.15.585257.
5
Breaking barriers in antibody discovery: harnessing divergent species for accessing difficult and conserved drug targets.打破抗体发现的障碍：利用多样化的物种来获得难以靶向和保守的药物靶点。
MAbs. 2023 Jan-Dec;15(1):2273018. doi: 10.1080/19420862.2023.2273018. Epub 2023 Dec 5.
6
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BMB Rep. 2015 Sep;48(9):489-94. doi: 10.5483/bmbrep.2015.48.9.120.
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