Kumar Vikash, Kumar Rahul, Gurusubramanian Guruswami, Rathore Saurabh Singh, Roy Vikas Kumar
Department of Biotechnology, Mahatma Gandhi Central University, Motihari, Bihar, India.
Department of Zoology, Mizoram University, Aizawl, Mizoram, India.
J Biochem Mol Toxicol. 2025 May;39(5):e70294. doi: 10.1002/jbt.70294.
Di (2-ethylhexyl) phthalate (DEHP) is a plasticiser used in plastic products; and it dissolves easily and leaks into the environment. Due to its lipophilic nature, DEHP accumulates in organisms and can bioaccumulate through food chains. The natural flavonoid, like morin hydrate, possesses various pharmacological properties, including anti-inflammatory, antioxidant, and free radical scavenging. The purpose of this study was to investigate the effect of morin hydrate (MH) on kidney function of DEHP-treated mice. To investigate the underlying processes of the proposed objective, DEHP (500 mg/kg) and DEHP, along with MH at doses of 10 and 100 mg/kg, were administered to Swiss albino mice for 14 days. Our results showed that MH treatment improved kidney function by decreasing creatinine and urea levels in DEHP-intoxicated mice. Furthermore, the MH also alleviates DEHP-induced kidney fibrosis and kidney histoarchitecture. DEHP-mediated oxidative stress and stimulated apoptosis in the kidney were also mitigated by MH treatment. The elevated expression of NF-kB/TNF-α by the DEHP treatment was also down-regulated by the MH treatment. In addition, the abundance of HSP70 increased in the kidneys after DEHP treatment, and MH treatment also decreased the abundance of HSP70 in the kidneys. In conclusion, DEHP treatment caused kidney toxicity in mice, and MH mitigates the kidney functions via modulating NF-kB/TNF-α/oxidative stress/apoptosis pathway. Our findings provide the new findings that MH protects the kidneys from DEHP intoxication, highlighting the potential of MH as a protective treatment for DEHP toxicity and offering hope for future research and treatments.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种用于塑料制品的增塑剂；它很容易溶解并泄漏到环境中。由于其亲脂性，DEHP会在生物体内蓄积，并可通过食物链进行生物累积。天然黄酮类化合物，如水合桑色素，具有多种药理特性，包括抗炎、抗氧化和清除自由基。本研究的目的是探讨水合桑色素（MH）对DEHP处理小鼠肾功能的影响。为了研究所提出目标的潜在过程，将DEHP（500mg/kg）以及10和100mg/kg剂量的DEHP与MH一起给予瑞士白化小鼠，持续14天。我们的结果表明，MH治疗通过降低DEHP中毒小鼠的肌酐和尿素水平改善了肾功能。此外，MH还减轻了DEHP诱导的肾纤维化和肾脏组织结构损伤。DEHP介导的肾脏氧化应激和细胞凋亡也通过MH治疗得到缓解。DEHP处理导致的NF-κB/TNF-α表达升高也通过MH治疗下调。此外，DEHP处理后肾脏中HSP70的丰度增加，而MH治疗也降低了肾脏中HSP70的丰度。总之，DEHP处理导致小鼠肾脏毒性，而MH通过调节NF-κB/TNF-α/氧化应激/细胞凋亡途径减轻肾功能损害。我们的研究结果提供了新的发现，即MH可保护肾脏免受DEHP中毒，突出了MH作为DEHP毒性保护治疗的潜力，并为未来的研究和治疗带来了希望。
