Examining the relationship between persistent organic pollutants and metabolic-associated fatty liver disease: a cross-sectional analysis using NHANES data.

Recently, the term Metabolic-Associated Fatty Liver Disease (MAFLD) has been adopted to better reflect the underlying pathology and association with metabolic issues. Beyond dietary factors and physical activity, previous studies have suggested that persistent organic pollutants (POPs) may contribute to the etiology of MAFLD; however, this disease can also develop at very low POP exposure levels, making it challenging to discern their specific effect. This study aims to investigate the potential link between exposure to POPs and the prevalence of MAFLD. Data from the National Health and Nutrition Examination Survey (NHANES) was utilized for this cross-sectional study. Participants were categorized based on their MAFLD status and levels of various POPs measured in their blood serum. Cox regression to estimate adjusted prevalence ratio (aPR) of MAFLD was used. Hazard Index (HI), Proportion of Maximum Scaling (POMS), and Toxicity Burden Index (TBI) were applied to assess exposure to mixtures. A total of 4,224 participants were included, 47 (33-65) years, 53.0% were women, and 50.1% had MAFLD. No significant sex differences were observed in the main analysis regarding the association between individual POPs and MAFLD prevalence. However, sensitivity analyses revealed an inverse relationship between certain POPs and MAFLD prevalence, particularly in women. Higher levels of specific PCBs were associated with a lower prevalence of MAFLD in women. This study highlighted the effects of individual pollutants, mixtures, and sex-specific differences. The combined use of HI, POMS, and TBI provided a more detailed risk assessment. Findings suggest that biological sex and metabolic stressors play significant roles in how POPs influence MAFLD, warranting further investigation into mechanisms and health outcomes in different exposure ranges.