Murray M, Hirumi H, Moloo S K
Parasitology. 1985 Aug;91 ( Pt 1):53-66. doi: 10.1017/s0031182000056511.
Significant suppression in the incidence of cyclical development of Trypanosoma congolense, T. vivax and T. brucei occurred in Glossina morsitans centralis maintained on goats immunized with in vitro-propagated uncoated forms of T. congolense, T. vivax and T. brucei, respectively. This was observed when tsetse given a T. congolense-infected feed were subsequently maintained on uninfected immunized goats and also when uninfected tsetse were fed on immunized goats infected with T. congolense, T. vivax and T. brucei. Suppression of infection rates in tsetse was trypanosome species specific, but was independent of the trypanosome stock used for immunization of goats. These findings were reflected in antibody responses to uncoated trypanosomes, as measured by immunofluorescence and the solid-phase immunoradiometric binding assay. Thus, antibody from goats immunized with uncoated trypanosomes of one species exhibited minimal reactivity with uncoated forms of other species of trypanosomes, but showed high levels of activity with uncoated forms of the same or unrelated stocks of the same species. However, in view of the range of hosts upon which tsetse feed, it is open to question whether the use of a vaccine which suppresses trypanosome infection rates in tsetse would have any significant effect in the field.
分别用体外增殖的无包被形式的刚果锥虫、活跃锥虫和布氏锥虫免疫山羊，然后以这些山羊为食源饲养的中喙采采蝇，其体内刚果锥虫、活跃锥虫和布氏锥虫的周期性发育发生率显著降低。当给采采蝇投喂感染刚果锥虫的饲料后，再将其饲养在未感染的免疫山羊上，以及当未感染的采采蝇取食感染刚果锥虫、活跃锥虫和布氏锥虫的免疫山羊时，均观察到这种情况。采采蝇感染率的降低具有锥虫物种特异性，但与用于免疫山羊的锥虫株无关。这些发现反映在用免疫荧光和固相免疫放射结合试验检测的对无包被锥虫的抗体反应中。因此，用一种锥虫的无包被形式免疫的山羊产生的抗体，与其他物种锥虫的无包被形式反应性极低，但与同一物种相同或无关株系的无包被形式反应活性很高。然而，鉴于采采蝇取食的宿主范围，在采采蝇中抑制锥虫感染率的疫苗在野外是否会有任何显著效果仍值得怀疑。
