Aghazadeh Ghadim Mir Behrad, Salimi-Sabour Ebrahim, Shahriari Alireza, Niazi Mahdi, Bahrami Farideh
Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Physiology and Medical Physics, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Iran J Basic Med Sci. 2025;28(6):746-754. doi: 10.22038/ijbms.2025.82718.17878.
Sleep impacts the well-being and quality of life of millions. Given conventional pharmacotherapy's limitations and side effects, the quest for adequate and proper sleep promotion is imperative. This study aims to identify a suitable and effective compound for sleep by examining qualified herbal compounds in the PubChem database using methods. Ultimately, the extracted compound (ginkgetin, a bioactive flavonoid from ) through molecular docking by considering the GABAA receptors will be evaluated through the method in an animal model to serve as proof for the findings from the molecular docking process.
Utilizing a comprehensive approach, this research employed molecular docking to screen 2299 phytochemicals for their affinity towards the GABAA receptor, focusing on the GABA, benzodiazepine, and steroid-binding sites. Ginkgetin emerged as a top candidate due to its high binding affinity. Subsequent electrophysiological assessments in rats treated with extract containing ginkgetin evaluated alterations in sleep architecture, REM, and NREM sleep phases.
Molecular docking identified ginkgetin as possessing the highest binding affinity among the screened phytochemicals. studies corroborated these findings, demonstrating that rats treated with extract significantly enhanced REM and NREM sleep compared to controls.
Ginkgetin, derived from , shows promising potential as a novel therapeutic agent for sleep disorders, supported by its strong affinity to key receptor sites and its efficacy in modulating sleep architecture . These findings contribute to the expanding evidence base for the therapeutic use of in sleep promotion and underscore the need for further research to elucidate the mechanisms and clinical applicability of ginkgetin in sleep disorder treatment.
睡眠影响着数百万人的幸福感和生活质量。鉴于传统药物治疗的局限性和副作用，寻求适当且合适的促进睡眠方法势在必行。本研究旨在通过使用相关方法检查PubChem数据库中的合格草药化合物，来确定一种适合且有效的促进睡眠化合物。最终，通过考虑GABAA受体的分子对接提取出的化合物（银杏双黄酮，一种来自[原文此处缺失相关植物名称]的生物活性黄酮类化合物）将在动物模型中通过[原文此处缺失相关方法名称]方法进行评估，以作为分子对接过程结果的证据。
本研究采用综合方法，利用分子对接技术筛选2299种植物化学物质对GABAA受体的亲和力，重点关注GABA、苯二氮䓬和类固醇结合位点。由于银杏双黄酮具有高结合亲和力，它成为了顶级候选物。随后，对用含银杏双黄酮提取物处理的大鼠进行电生理评估，以评估睡眠结构、快速眼动（REM）和非快速眼动（NREM）睡眠阶段的变化。
分子对接确定银杏双黄酮在筛选出的植物化学物质中具有最高的结合亲和力。[原文此处缺失相关研究名称]研究证实了这些发现，表明与对照组相比，用[原文此处缺失相关提取物名称]提取物处理的大鼠显著增加了快速眼动和非快速眼动睡眠。
源自[原文此处缺失相关植物名称]的银杏双黄酮，因其对关键受体位点的强亲和力及其调节睡眠结构的功效，显示出作为睡眠障碍新型治疗剂的潜在前景。这些发现为[原文此处缺失相关植物名称]在促进睡眠治疗中的应用提供了越来越多的证据，并强调需要进一步研究以阐明银杏双黄酮在睡眠障碍治疗中的机制和临床适用性。
