Lee Kangwook, Choi La Yoon, Ahn Jun Sang, Song Jae Yong, Park Joong Kook, Yun Suk Jun, Lee Jeong Heon, Shin Eui-Cheol, Yeom Soo-Jin, Zhao Jiangchao, Cho Tae Jin, Oh Nam Su, Shin Jeong-Oh, Kim Dahye, Kim Tae Gyun, Cho Hyung Taek, Shin Hyo Ri, Kim Young Jun, Kim Jae Kyeom
Department of Food and Biotechnology, Korea University, Sejong, Republic of Korea.
Nonghyup Feed, Seoul, Republic of Korea.
Front Vet Sci. 2025 Apr 24;12:1546248. doi: 10.3389/fvets.2025.1546248. eCollection 2025.
The present study investigated the effects of antioxidant supplementation on the transcriptomic profiles of cattle during a 7-month feeding trial.
Twelve castrated cattle were randomly assigned to two groups: a control group (CON) and a group supplemented with antioxidants (FEED), consisting of vitamin C, vitamin E, and selenium. Growth performance and carcass traits were evaluated, and liver transcriptomic changes were assessed using RNA sequencing.
While no significant differences were observed in phenotypic traits such as weight gain and feed conversion ratio, transcriptomic analysis identified 641 differentially expressed genes between the CON and FEED groups. Functional enrichment analysis revealed that differentially expressed genes were mainly associated with transcription regulation, pseudouridine synthesis, and mitochondrial function. These findings suggest that antioxidant supplementation elicits significant molecular changes in the liver, particularly affecting transcriptional activity and mitochondrial processes, even in the absence of detectable phenotypic differences.
本研究在一项为期7个月的饲养试验中，调查了补充抗氧化剂对牛转录组图谱的影响。
将12头去势公牛随机分为两组：对照组（CON）和补充抗氧化剂组（FEED），后者由维生素C、维生素E和硒组成。评估生长性能和胴体性状，并使用RNA测序评估肝脏转录组变化。
虽然在体重增加和饲料转化率等表型性状上未观察到显著差异，但转录组分析确定了CON组和FEED组之间有641个差异表达基因。功能富集分析表明，差异表达基因主要与转录调控、假尿苷合成和线粒体功能有关。这些发现表明，即使在没有可检测到的表型差异的情况下，补充抗氧化剂也会在肝脏中引起显著的分子变化，特别是影响转录活性和线粒体过程。
