Orbán E C, Pallos J P, Kocsár L T
Acta Med Hung. 1985;42(1-2):77-84.
Sensitive tritiated radioimmunoassay was developed for conjugated chenodeoxycholic acid, using immunogen prepared by the mixed anhydride method. The obtained molar bile salt-BSA ratio in the immunogen was 19:1. The distinguishing features of the immunization procedure were a preliminary vaccination of the animal with antitubercular vaccine (VDS), and the administration of very small doses of immunogen (50 micrograms). Assay sensitivity for this bile salt fell in the picomole range with the standard curve extending from 1.5 to 150 pmol. Specificity of the antiserum was compared with that of the commercially available "Glycochenodeoxycholic acid RIA kit" (Nordiclab Oy, Oulu, Finland), and proved to be satisfactory. Fasting serum conjugated chenodeoxycholic acid concentration in 25 healthy subjects and 15 patients with cirrhosis was 0.63 mumol/l and 43.05 mumol/l, respectively. The assay was performed on unextracted sera.
采用混合酸酐法制备免疫原，建立了灵敏的氚标记放射免疫分析法测定结合型鹅去氧胆酸。免疫原中获得的胆盐与牛血清白蛋白的摩尔比为19:1。免疫程序的显著特点是先用抗结核疫苗（VDS）对动物进行初步接种，并给予极少量的免疫原（50微克）。该胆盐的检测灵敏度在皮摩尔范围内，标准曲线范围为1.5至150皮摩尔。将抗血清的特异性与市售的“甘氨鹅去氧胆酸放射免疫分析试剂盒”（芬兰奥卢市Nordiclab Oy公司）进行比较，结果令人满意。25名健康受试者和15名肝硬化患者的空腹血清结合型鹅去氧胆酸浓度分别为0.63微摩尔/升和43.05微摩尔/升。该分析在未提取的血清上进行。
