通过超高效液相色谱-串联质谱技术对表观遗传DNA和RNA修饰进行定量分析：特殊样本的新概念与新进展
Quantification of Epigenetic DNA and RNA Modifications by UHPLC-MS/MS Technologies: New Concepts and New Improvements for the Special Collections.
作者信息
Zhang Rui, Liu Hailong, Bai Biao, Wang Hailin
机构信息
The State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
出版信息
J Sep Sci. 2025 May;48(5):e70159. doi: 10.1002/jssc.70159.
Dynamic and reversible DNA and RNA modifications are essential for cell differentiation and development. Aberrant epigenetic modifications are closely associated with the occurrence and progression of diseases, serving as potential markers for cancer diagnosis and prognosis. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) offers distinct advantages in the qualitative and quantitative analysis of various modifications due to its sensitivity, specificity, and accuracy. This review provides a comprehensive overview of the current knowledge regarding the liquid chromatography-mass spectrometry (LC-MS) analysis of DNA and RNA modifications, including analytical procedures, advancements, and biological applications, with a focus on tracing the source of (N6-2'-deoxy-adenosine) 6mdA in eukaryotes. Additionally, we examine the integration of UHPLC-MS/MS with other separation techniques to achieve accurate quantification of modifications in specific regions, certain fragments, and free nucleosides.
动态且可逆的DNA和RNA修饰对于细胞分化和发育至关重要。异常的表观遗传修饰与疾病的发生和进展密切相关，可作为癌症诊断和预后的潜在标志物。超高效液相色谱-串联质谱联用技术（UHPLC-MS/MS）因其灵敏度、特异性和准确性，在各种修饰的定性和定量分析中具有显著优势。本综述全面概述了当前关于DNA和RNA修饰的液相色谱-质谱（LC-MS）分析的知识，包括分析程序、进展和生物学应用，重点是追踪真核生物中（N6-2'-脱氧腺苷）6mdA的来源。此外，我们还研究了UHPLC-MS/MS与其他分离技术的整合，以实现对特定区域、某些片段和游离核苷中修饰的准确定量。
Zotero 插件