一个C57 BL/6J小鼠的多组学数据集，通过用ε-聚赖氨酸喂养来调节功能。

A multi-omics dataset of C57 BL/6J mice regulated function by feed with ε-polylysine.

作者信息

Zhang Xuelei, Hou Zhenping, Cao Yuxin, Wang Songshan, Zhang Yong, Wang Jinrong, Wu Duanqin

机构信息

School of Biological Engineering, Henan University of technology, Zhengzhou, China.

Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China.

出版信息

Sci Data. 2025 May 10;12(1):771. doi: 10.1038/s41597-025-05103-8.

DOI:10.1038/s41597-025-05103-8

PMID:40348754

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12065851/

Abstract

The proteomic and metabolomic differences in mice fed ε-polylysine were detected by high-throughput sequencing and screening. The effects of ε-polylysine on the proteome and metabolomics of C57 BL/6 J mice were studied. DIA proteome sequencing and liquid chromatography-mass spectrometry (LC-MS) were used to compare ε-polylysine-modified mouse proteome and metabolome. In this study, we analyzed the regulatory effects of ε-polysine on glycerophospholipid metabolism, glutathione metabolism, and ABC transporters pathways. The sequencing data and LC-MS/MS raw data of samples are stored in MetaboLights and ProteomeXchange databases for efficient reuse and to explore the differences in metabolism and protein expression among mice fed with different levels of ε-polylysine, aiming to screen for critical proteins and differential metabolites in valuable regulatory pathways.

摘要

通过高通量测序和筛选检测喂食ε-聚赖氨酸的小鼠的蛋白质组学和代谢组学差异。研究了ε-聚赖氨酸对C57 BL/6 J小鼠蛋白质组和代谢组学的影响。采用数据独立采集（DIA）蛋白质组测序和液相色谱-质谱联用（LC-MS）比较ε-聚赖氨酸修饰的小鼠蛋白质组和代谢组。在本研究中，我们分析了ε-聚赖氨酸对甘油磷脂代谢、谷胱甘肽代谢和ABC转运蛋白途径的调节作用。样本的测序数据和LC-MS/MS原始数据存储在MetaboLights和ProteomeXchange数据库中，以便有效重复使用，并探索不同水平ε-聚赖氨酸喂养的小鼠之间的代谢和蛋白质表达差异，旨在筛选有价值的调控途径中的关键蛋白质和差异代谢物。