Zhu Haotian, Jiang Kaixuan, Meng Jingjing, Kuang Lulu, Zhu Shuang, Zhang Yongji, Wang Youping, Jiang Jinjin
Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Saline-Alkali Soil Reclamation and Utilization in Coastal Areas, the Ministry of Agriculture and Rural Affairs of China, Yangzhou 225009, China.
Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China.
Plant Sci. 2025 Aug;357:112523. doi: 10.1016/j.plantsci.2025.112523. Epub 2025 May 9.
Brassica napus L. is an important oil crop grown worldwide. Mining for genes related to abiotic stress tolerance is valuable to improve the adaptability and increase the cultivation of B. napus. miR393 is a conserved miRNA family that plays multiple roles in regulating plant growth, development, and response to biotic and abiotic stresses. In this study, we reported miR393 as a positive regulator of anthocyanin biosynthesis that improved rapeseed tolerance to oxidative and osmotic stresses. Overexpression of miR393 up-regulated the expression of anthocyanin biosynthetic genes and stress responsive genes, thus resulted more anthocyanin accumulation and improved ROS-scavenging capability. Overall, this study indicated that miR393 could be applied in genetic improvement of B. napus with osmotic stress tolerance.
甘蓝型油菜是一种在全球广泛种植的重要油料作物。挖掘与非生物胁迫耐受性相关的基因对于提高甘蓝型油菜的适应性和扩大其种植面积具有重要价值。miR393是一个保守的miRNA家族，在调节植物生长、发育以及对生物和非生物胁迫的响应中发挥多种作用。在本研究中，我们报道miR393作为花青素生物合成的正调控因子，可提高油菜对氧化和渗透胁迫的耐受性。miR393的过表达上调了花青素生物合成基因和胁迫响应基因的表达，从而导致更多花青素积累并提高了活性氧清除能力。总体而言，本研究表明miR393可应用于甘蓝型油菜耐渗透胁迫的遗传改良。
