血蓝蛋白的岩藻糖基化对凡纳滨对虾的抗菌免疫至关重要。
Fucosylation of hemocyanin is critical for antibacterial immunity in Penaeus vannamei.
作者信息
Jiang Mingming, Liu Yiqi, Aweya Jude Juventus, Liang Shuaiqi, Zhou Junyang, Tayyab Muhammad, Zhao Yongzhen, Liu Qingyun, Zhang Yueling, Zheng Zhihong
机构信息
Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, 515063, China.
Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, 515063, China; Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, Canada; The Canadian Centre for Agri-Food Research in Health and Medicine, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, MB, Canada.
出版信息
Fish Shellfish Immunol. 2025 Aug;163:110406. doi: 10.1016/j.fsi.2025.110406. Epub 2025 May 9.
Posttranslational modifications (PTMs) play a pivotal role in expanding the function of immune related proteins, especially during pathogen infections. However, the precise impact of PTMs on the functional diversity of proteins such as respiratory glycoproteins and hemocyanins remains incompletely understood. In this study, we investigated the fucosylation modification of Penaeus vannamei hemocyanin (PvHMC) and its impact on antibacterial immunity. Our findings reveal that PvHMC underwent fucosylation, mediated by Penaeus vannamei fucosyltransferase 8/10 (PvFUT8/10), which enhances its antibacterial immunity and binding ability against Gram-negative bacteria. Conversely, defucosylation of PvHMC, catalyzed by Penaeus vannamei fucosidase (PvAFU) weakens its antibacterial activity in vitro. These results highlight the regulatory role of fucosylation in modulating the antibacterial function of PvHMC in shrimp.
翻译后修饰（PTMs）在扩展免疫相关蛋白的功能方面发挥着关键作用，尤其是在病原体感染期间。然而，PTMs对呼吸糖蛋白和血蓝蛋白等蛋白质功能多样性的确切影响仍未完全了解。在本研究中，我们研究了凡纳滨对虾血蓝蛋白（PvHMC）的岩藻糖基化修饰及其对抗菌免疫的影响。我们的研究结果表明，PvHMC在凡纳滨对虾岩藻糖基转移酶8/10（PvFUT8/10）的介导下发生岩藻糖基化，这增强了其抗菌免疫力以及对革兰氏阴性菌的结合能力。相反，凡纳滨对虾岩藻糖苷酶（PvAFU）催化的PvHMC去岩藻糖基化作用会削弱其体外抗菌活性。这些结果突出了岩藻糖基化在调节虾中PvHMC抗菌功能方面的调节作用。
Zotero 插件