具有铜离子耐受性的新型噬菌体vB_Mm5
New Phage vB_Mm5 with Tolerance to Cu Ions.
作者信息
Goliadze Rusudan, Kamashidze Luka, Karumidze Natia, Rigvava Sophio, Rcheulishvili Olia, Rcheulishvili Aleksander, Goderdzishvili Marine, Kusradze Ia
机构信息
Laboratory of General Microbiology, George Eliava Institute of Bacteriophages, Microbiology and Virology, Tbilisi, Georgia.
Faculty of Medicine, European University, Tbilisi, Georgia.
出版信息
Phage (New Rochelle). 2025 Mar 17;6(1):41-47. doi: 10.1089/phage.2024.0036. eCollection 2025 Mar.
BACKGROUND
Research on phages targeting is still emerging, with limited studies compared with other phage-host systems. Interest in these phages has increased due to rising antibiotic resistance and their potential for controlling spread in the environment.
MATERIALS AND METHODS
This study investigates the biology and genetics of the novel -infecting myophage and evaluates its stability under Cu exposure.
RESULTS
Phage has a 10-min latent period and a burst size of 30 (±5). It shows high tolerance to elevated temperatures and Cu. The phage genome, comprising 163,232 bp dsDNA with 229 open reading frames, encodes genes that not only enhance the phage's predatory capabilities but also confer resistance to host defense mechanisms.
CONCLUSIONS
is highly distinct from other sequenced phages, does not contain any known virulence genes, and holds potential as a therapeutic agent against infections.
背景
针对[具体目标菌]的噬菌体研究仍在不断涌现，与其他噬菌体 - 宿主系统相比，相关研究有限。由于抗生素耐药性的上升以及它们在控制[具体目标菌]在环境中传播的潜力，人们对这些噬菌体的兴趣有所增加。
材料与方法
本研究调查了新型感染[具体目标菌]的肌尾噬菌体[噬菌体名称]的生物学和遗传学特性，并评估了其在铜暴露下的稳定性。
结果
噬菌体[噬菌体名称]的潜伏期为10分钟，裂解量为30（±5）。它对高温和铜具有高度耐受性。噬菌体基因组由163,232 bp的双链DNA组成，含有229个开放阅读框，编码的基因不仅增强了噬菌体的捕食能力，还赋予了对宿主防御机制的抗性。
结论
[噬菌体名称]与其他已测序的[具体目标菌]噬菌体高度不同，不包含任何已知的毒力基因，具有作为抗[具体目标菌]感染治疗剂的潜力。
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