六种新型噬菌体：基因组见解与治疗潜力
Six Novel Phages: Genomic Insights and Therapeutic Potential.
作者信息
Rimon Amit, Yerushalmy Ortal, Belin Jonathan, Alkalay-Oren Sivan, Gavish Lilach, Coppenhagen-Glazer Shunit, Hazan Ronen
机构信息
Faculty of Dental Medicine, Institute of Biomedical and Oral Research (IBOR), The Hebrew University of Jerusalem, Jerusalem, Israel.
Tzameret, The Military Track of Medicine, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
出版信息
Phage (New Rochelle). 2025 Mar 17;6(1):32-40. doi: 10.1089/phage.2024.0037. eCollection 2025 Mar.
INTRODUCTION
is an opportunistic pathogen that causes health care-associated infections. The rise of antibiotic-resistant bacterial strains necessitates alternative treatment strategies, with bacteriophage therapy being a promising approach.
METHODS
Six bacteriophages were isolated from sewage samples. Phage isolation involved centrifugation, filtration, and plaque assays. The morphology of each sample was examined using transmission electron microscopy (TEM). Genomic DNA was sequenced and compared among the isolates. The phages' lytic activities were assessed using growth curve analysis.
RESULTS
The phages displayed distinct genomic characteristics, grouping into three genomic clusters. No known virulence or antibiotic resistance genes were detected, indicating their safety for therapeutic use. Taxonomic analysis identified the phages as belonging to the genera , , , and a novel genus. TEM analysis revealed their diverse morphologies. Temperate phages showed less effective lytic activities.
CONCLUSION
Several of the isolated bacteriophages show potential as candidates for phage therapy research and could be effective against infections.
引言
是一种引起医疗保健相关感染的机会致病菌。抗生素耐药菌株的增加使得替代治疗策略成为必要，噬菌体疗法是一种有前景的方法。
方法
从污水样本中分离出六种噬菌体。噬菌体分离包括离心、过滤和噬菌斑测定。使用透射电子显微镜（TEM）检查每个样本的形态。对分离株的基因组DNA进行测序并比较。使用生长曲线分析评估噬菌体的裂解活性。
结果
噬菌体表现出不同的基因组特征，分为三个基因组簇。未检测到已知的毒力或抗生素抗性基因，表明它们用于治疗是安全的。分类学分析确定这些噬菌体属于属、属、属和一个新属。TEM分析揭示了它们多样的形态。温和噬菌体的裂解活性较低。
结论
几种分离出的噬菌体显示出作为噬菌体疗法研究候选物的潜力，并且可能对感染有效。
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