Kairupan Tara S, Kapantow Nova H, Tallei Trina E, Niode Nurdjannah J, Sanggelorang Yulianty, Rotty Linda Wa, Wungouw Herlina Is, Kawengian Shirley Es, Fatimawali Fatimawali, Maulydia Nur B
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Sam Ratulangi, Manado, Indonesia.
Department of Dermatology and Venereology, Faculty of Medicine, Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia.
Narra J. 2025 Apr;5(1):e1185. doi: 10.52225/narra.v5i1.1185. Epub 2025 Feb 3.
Marine-derived collagen peptides have been acknowledged for their therapeutic potential, especially in cancer therapy and inflammation management. The aim of this study was to investigate the molecular mechanisms that contribute to the anticancer, anti-inflammatory and antioxidant properties of yellowfin tuna collagen peptides (YFTCP) utilizing a network pharmacology approach. The YFTCP was extracted from the bones of yellowfin tuna and subsequently hydrolyzed with trypsin. Seventeen peptides were discovered using liquid chromatography in conjunction with high-resolution mass spectrometry (LC-HRMS). A network pharmacology method was utilized to investigate the interactions between the discovered peptides and their biological targets. Additionally, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to identify pertinent biological pathways involved in the anticancer, antioxidant, and anti-inflammatory effects of these peptides. GO analysis revealed key associations between YFTCP and critical cancer- and inflammation-related genes encoding proteins such as CCND1, SRC, AKT, IL-1β, TNF, and PPARG, which exhibited significant interactions. These proteins are essential for the regulation of the cell cycle, the development of tumors, and the response to inflammatory stimuli. The KEGG analysis also revealed that YFTCP was involved in a number of critical pathways, such as endocrine resistance, cancer pathways, Kaposi sarcoma-associated herpesvirus infection, proteoglycans in cancer, and human cytomegalovirus infection. These findings highlight the potential use of YFTCP as a multifaceted therapeutic agent, indicating their role in regulating important biological pathways associated with cancer development and inflammation. This study provides new valuable insights into the pharmacological properties of YFTCP, paving the way for future studies and drug development focused on these bioactive peptides.
海洋来源的胶原蛋白肽因其治疗潜力而受到认可，尤其是在癌症治疗和炎症管理方面。本研究的目的是利用网络药理学方法，研究有助于黄鳍金枪鱼胶原蛋白肽（YFTCP）发挥抗癌、抗炎和抗氧化特性的分子机制。YFTCP是从黄鳍金枪鱼的骨骼中提取的，随后用胰蛋白酶进行水解。使用液相色谱结合高分辨率质谱（LC-HRMS）发现了17种肽。利用网络药理学方法研究发现的肽与其生物学靶点之间的相互作用。此外，还进行了基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析，以确定这些肽的抗癌、抗氧化和抗炎作用所涉及的相关生物学途径。GO分析揭示了YFTCP与关键的癌症和炎症相关基因之间的关键关联，这些基因编码的蛋白质如CCND1、SRC、AKT、IL-1β、TNF和PPARG，它们之间表现出显著的相互作用。这些蛋白质对于细胞周期的调节、肿瘤的发展以及对炎症刺激的反应至关重要。KEGG分析还表明，YFTCP参与了许多关键途径，如内分泌抵抗、癌症途径、卡波西肉瘤相关疱疹病毒感染、癌症中的蛋白聚糖以及人巨细胞病毒感染。这些发现突出了YFTCP作为一种多方面治疗剂的潜在用途，表明它们在调节与癌症发展和炎症相关的重要生物学途径中的作用。本研究为YFTCP的药理特性提供了新的有价值的见解，为未来针对这些生物活性肽的研究和药物开发铺平了道路。
