Shim Yerin, Cho Minji, Kim Misook
Major in Nutrition Education, Graduate School of Education, Dankook University, Gyeonggi 16890, Korea.
Department of Food Science and Nutrition, Dankook University, Chungnam 31116, Korea.
Prev Nutr Food Sci. 2025 Apr 30;30(2):196-206. doi: 10.3746/pnf.2025.30.2.196.
In this study, functional yogurt enriched with L. Osbeck (CS) powder was prepared, and its fermentation characteristics, antioxidant activity, and inhibition properties against enzymes related to diabetes and obesity treatments, namely α-amylase, α-glucosidase, and lipase, were investigated. The yogurt was prepared by adding 1%, 3%, and 5% CS powder to skim milk, followed by sterilization and fermentation with a mixed starter culture for 6 h at 39°C. After fermentation, the yogurt had viable cell counts between 8.05 and 8.21 log colony-forming units/mL and a pH reduction to 4.54-4.63. The total anthocyanin content increased with increasing concentrations of CS powder. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity and ferric reducing antioxidant power values also improved, with the highest antioxidant activity observed in the yogurt prepared with 5% CS powder. With regard to enzyme activity inhibition, CS addition significantly increased the α-glucosidase inhibitory activity, whereas the α-amylase inhibitory activity remained unchanged. These findings suggest that yogurt fortified with CS powder exhibits improved antioxidant activity and selective α-glucosidase inhibition, making it a promising candidate for the preparation of functional fermented beverages.
在本研究中,制备了富含欧李(CS)粉的功能性酸奶,并研究了其发酵特性、抗氧化活性以及对与糖尿病和肥胖症治疗相关的酶(即α-淀粉酶、α-葡萄糖苷酶和脂肪酶)的抑制特性。通过向脱脂乳中添加1%、3%和5%的CS粉来制备酸奶,随后进行灭菌,并在39°C下用混合发酵剂培养物发酵6小时。发酵后,酸奶的活菌计数在8.05至8.21 log菌落形成单位/毫升之间,pH值降至4.54 - 4.63。总花青素含量随着CS粉浓度的增加而增加。1,1-二苯基-2-苦基肼自由基清除活性和铁还原抗氧化能力值也有所提高,在添加5% CS粉制备的酸奶中观察到最高的抗氧化活性。关于酶活性抑制,添加CS显著提高了α-葡萄糖苷酶抑制活性,而α-淀粉酶抑制活性保持不变。这些发现表明,添加CS粉的酸奶具有改善的抗氧化活性和选择性α-葡萄糖苷酶抑制作用,使其成为制备功能性发酵饮料的有前景的候选物。