Shramova E I, Proshkina G M, Deyev S M
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
National Research Center "Kurchatov Institute,", Moscow, Russia.
Dokl Biochem Biophys. 2025 May 11. doi: 10.1134/S1607672925700188.
Intravital bioimaging based on luminescence is an important method for the development and testing of antitumor drugs on model animals and is an essential part of preclinical studies. Bioimaging based on luminescent systems, compared with fluorescent bioimaging, provides a high signal-to-noise ratio, which justifies the development of cell lines that stably express luciferase genes for their subsequent use in model animals. In this work, we describe the creation of a stable cell line SKOV3.ip1-NanoLuc constitutively expressing the NanoLuc luciferase gene. The developed cell line was shown to be effective for intravital luminescence bioimaging of immunodeficient animals with deep-seated intraperitoneal tumors, which can be considered as a model of late-stage ovarian cancer.
基于发光的活体生物成像技术是在模型动物上开发和测试抗肿瘤药物的重要方法，也是临床前研究的重要组成部分。与荧光生物成像相比，基于发光系统的生物成像具有较高的信噪比，这为稳定表达荧光素酶基因的细胞系的开发提供了依据，以便随后在模型动物中使用。在这项工作中，我们描述了一个稳定表达纳米荧光素酶基因的细胞系SKOV3.ip1-NanoLuc的创建。所开发的细胞系被证明可有效地用于对患有深部腹腔肿瘤的免疫缺陷动物进行活体发光生物成像，这种动物模型可被视为晚期卵巢癌模型。
