HIV-1整合酶的新型细胞伴侣及其在病毒复制中的作用

New Cellular Partners of HIV-1 Integrase and their Role in Viral Replication.

作者信息

Agapkina Y Y, Ponomareva T Y, Vdovina M V, Ziganshin R H, Rozina A A, Anisenko A N, Gottikh M B

机构信息

Lomonosov Moscow State University, Department of Chemistry, Moscow, Russia.

Lomonosov Moscow State University, Belozersky Research Institute of Physico-Chemical Biology, Moscow, Russia.

出版信息

Dokl Biochem Biophys. 2025 May 11. doi: 10.1134/S1607672925600149.

DOI:10.1134/S1607672925600149

PMID:40353973

Abstract

Cellular proteins, partners of viral enzymes, are involved in the replication of human immunodeficiency virus type I (HIV-1) at various stages. Thus, the viral enzyme integrase, participating in several stages of the viral cycle, interacts with various cellular proteins. A number of them are already known, and for some the mechanism of action has been established, but the search for cellular partners of integrase continues. In this work, the identification of cellular partners of HIV-1 integrase has been carried out by the method of cross-linking followed by mass spectrometry (XL-MS). Twelve new potential integrase partners have been identified, and some of them have been examined for their effect on the early stages of HIV-1 replication.

摘要

细胞蛋白作为病毒酶的伙伴，在人类免疫缺陷病毒1型（HIV-1）复制的各个阶段发挥作用。因此，参与病毒周期多个阶段的病毒酶整合酶与多种细胞蛋白相互作用。其中一些细胞蛋白已为人所知，部分蛋白的作用机制也已明确，但对整合酶细胞伙伴的探寻仍在继续。在这项研究中，通过交联后质谱分析（XL-MS）的方法对HIV-1整合酶的细胞伙伴进行了鉴定。已鉴定出12个新的潜在整合酶伙伴，并对其中一些伙伴在HIV-1复制早期阶段的作用进行了研究。