Ma Jinping, Wu Daoyi, Xu Chang, He Qing, Wang Mingjin, Imran Muhammad, Nazar Mudassar, Li Kun
Bijie Academy of Agricultural Sciences, Bijie, 551799, China.
Bijie Academy of Agricultural Sciences, Bijie, 551799, China.
Microb Pathog. 2025 Aug;205:107696. doi: 10.1016/j.micpath.2025.107696. Epub 2025 May 10.
Colitis is a multifactorial inflammatory bowel disease (IBD) involving intestinal barrier dysfunction, immune dysregulation, oxidative stress, and microbiota imbalance. Lactobacillus salivarius (L. salivarius), a probiotic with antioxidative and anti-inflammatory properties, was evaluated in a mouse model of DSS-induced colitis. Treatment with L. salivarius significantly reduced weight loss, colon shortening, and disease activity index, while improving histopathological damage. DSS-induced colitis exhibited significant oxidative stress, evidenced by decreased total antioxidant capacity, increased malondialdehyde (MDA), and reduced activity of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). After the intervention of L. salivarius, MDA levels in colon tissues decreased significantly, while SOD and GSH-Px activities increased significantly. In DSS-induced colitis models, the expression of pro-inflammatory cytokines (such as TNF-α, IL-6, IL-1β) was significantly increased, while the expression of anti-inflammatory cytokine IL-10 was decreased. After the intervention of L. salivarius, the level of proinflammatory factors was significantly down-regulated, while the level of IL-10 was up-regulated. DSS treatment leads to a significant imbalance in the intestinal microbiota, characterized by an increase in the ratio of Firmicutes to Bacteroidetes (F/B ratio). The abundance of pathogenic bacteria (Alistipes, Candidatus_Soleaferrea, Frisingicoccus, Romboutsia, Streptococcus) increased, while beneficial bacteria (Anaerotruncus, Rikenella) decreased. After probiotic administration, the F/B ratio was restored significantly, and the abundance of important beneficial bacteria increased while the abundance of pathogenic bacteria decreased. These results suggest that L. salivarius alleviates DSS-induced colitis by enhancing antioxidant defense, regulating inflammatory responses, and restoring gut microbiota balance. This probiotic may offer a promising therapeutic strategy for managing ulcerative colitis (UC).
结肠炎是一种多因素炎症性肠病（IBD），涉及肠道屏障功能障碍、免疫失调、氧化应激和微生物群失衡。唾液乳杆菌（L. salivarius）是一种具有抗氧化和抗炎特性的益生菌，在葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型中进行了评估。用唾液乳杆菌治疗可显著减轻体重减轻、结肠缩短和疾病活动指数，同时改善组织病理学损伤。DSS诱导的结肠炎表现出显著的氧化应激，表现为总抗氧化能力降低、丙二醛（MDA）增加以及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）等抗氧化酶活性降低。唾液乳杆菌干预后，结肠组织中MDA水平显著降低，而SOD和GSH-Px活性显著增加。在DSS诱导的结肠炎模型中，促炎细胞因子（如TNF-α、IL-6、IL-1β）的表达显著增加，而抗炎细胞因子IL-10的表达降低。唾液乳杆菌干预后，促炎因子水平显著下调，而IL-10水平上调。DSS治疗导致肠道微生物群显著失衡，其特征是厚壁菌门与拟杆菌门的比例（F/B比）增加。病原菌（Alistipes、Candidatus_Soleaferrea、Frisingicoccus、Romboutsia、链球菌）的丰度增加，而有益菌（Anaerotruncus、Rikenella）减少。给予益生菌后，F/B比显著恢复，重要有益菌的丰度增加，而病原菌的丰度降低。这些结果表明，唾液乳杆菌通过增强抗氧化防御、调节炎症反应和恢复肠道微生物群平衡来减轻DSS诱导的结肠炎。这种益生菌可能为治疗溃疡性结肠炎（UC）提供一种有前景的治疗策略。
