Rahman Md Hafizur, Siddique Abu Bakkar, Zihadi Md Aseif Hossain, Soheb Ahmed S M, Sumon Md Sazzad Hossain, Ahmed Shihab
AMR Reference Laboratory (Research), Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh.
Department of Public Health and Informatics, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
Sci Rep. 2025 May 12;15(1):16419. doi: 10.1038/s41598-025-01417-0.
The study aimed to investigate multi-drug resistant (MDR) Escherichia coli (E. coli) in companion animals in Bangladesh, with a focus on the resistance profiles of isolates from non-food-producing animals. In 2023, the studied samples were from cats, dogs, and environmental sources linked with companion animal hospitals in Dhaka city, Bangladesh. E. coli was isolated using standard techniques and its antimicrobial resistance (AMR) was assessed against 23 antibiotics following the CLSI protocols. Metallo-beta-lactamase genes (bla and bla) and mobile genetic elements (class 1 integron) were detected by multiplex PCR. The overall prevalence of E. coli was 70%, 76% in cats, 65.71% in dogs, and 65.71% in the environmental samples. Cefuroxime exhibited the highest resistance at 25%, while imipenem and nitrofurantoin showed the highest sensitivity at 100%, followed by ceftazidime at 95%. MDR strains made up 38.10%, while 11.90% were extremely drug-resistant (XDR). Additionally, 29% of E. coli were extended-spectrum beta-lactamase (ESBL) producers. The prevalence and association among class 1 integron and the resistant genes including bla and bla were also notable. This highlights the complex AMR challenges in these settings, including the presence of class 1 integron-a key element involved in capturing and transferring antimicrobial resistance genes.
该研究旨在调查孟加拉国伴侣动物中的多重耐药性（MDR）大肠杆菌（E. coli），重点关注非食用动物分离株的耐药谱。2023年，研究样本来自孟加拉国达卡市的猫、狗以及与伴侣动物医院相关的环境来源。使用标准技术分离大肠杆菌，并按照CLSI协议对其针对23种抗生素的抗菌耐药性（AMR）进行评估。通过多重PCR检测金属β-内酰胺酶基因（bla和bla）和移动遗传元件（1类整合子）。大肠杆菌的总体患病率为70%，猫中为76%，狗中为65.71%，环境样本中为65.71%。头孢呋辛的耐药率最高，为25%，而亚胺培南和呋喃妥因的敏感性最高，为100%，其次是头孢他啶，为95%。多重耐药菌株占38.10%，而11.90%为极耐药（XDR）。此外，29%的大肠杆菌是超广谱β-内酰胺酶（ESBL）产生菌。1类整合子与包括bla和bla在内的耐药基因之间的患病率和关联也很显著。这凸显了这些环境中复杂的抗菌耐药性挑战，包括1类整合子的存在——这是一个参与捕获和转移抗菌耐药基因的关键元件。
