Abady Mariam M, Jeong Ji-Seon, Kwon Ha-Jeong
Organic Metrology Group, Division of Chemical and Material Metrology, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea.
Department of Bio-Analytical Science, University of Science and Technology, Daejeon, Republic of Korea.
Rapid Commun Mass Spectrom. 2025 Aug 30;39(16):e10064. doi: 10.1002/rcm.10064.
Dried blood spot (DBS) sampling for the therapeutic drug monitoring of antiepileptic drugs offers practical advantages, including minimal invasiveness and ease of collection. However, for precise therapeutic management, its accuracy and reliability in quantification need to be validated.
This study validates DBS sampling for the analysis of 11 antiepileptic drugs using liquid chromatography-tandem mass spectrometry (LC-MS/MS), overcoming the physicochemical challenges associated with DBS samples.
The acetonitrile-based DBS extraction method demonstrated high efficiency for the 11 antiepileptic drugs. Accuracy and precision within 6% were achieved in both intra- and inter-day assays, with good selectivity, minimal matrix effects, and negligible carryover. All antiepileptic drugs exhibited stability in DBS samples for at least 30 days at room temperature, confirming proper handling and storage of the DBS samples. A 3 mm diameter disc punched from a DBS produced accurate results for all target drugs.
The optimized method provided a time- and cost-effective solution, showing a strong correlation between drug concentrations in whole blood, thereby supporting the suitability of DBS sampling as a promising and advanced method for antiepileptic drug monitoring.
用于抗癫痫药物治疗药物监测的干血斑（DBS）采样具有实际优势，包括微创性和易于采集。然而，为了进行精确的治疗管理，其在定量分析中的准确性和可靠性需要得到验证。
本研究使用液相色谱 - 串联质谱法（LC-MS/MS）验证了用于分析11种抗癫痫药物的DBS采样方法，克服了与DBS样本相关的物理化学挑战。
基于乙腈的DBS提取方法对11种抗癫痫药物显示出高效性。日内和日间测定的准确度和精密度均在6%以内，具有良好的选择性、最小的基质效应和可忽略的残留。所有抗癫痫药物在室温下于DBS样本中至少稳定30天，证实了DBS样本的妥善处理和储存。从DBS上冲压出的直径为3毫米的圆片对所有目标药物均产生了准确的结果。
优化后的方法提供了一种具有时间和成本效益的解决方案，显示全血中药物浓度之间具有很强的相关性，从而支持DBS采样作为一种有前景的先进抗癫痫药物监测方法的适用性。
