Zhan Xiangyang, Wang Wenkai, Lian Jie, Li Yichun, Gu Jianyi, Guo Dongdong, Xu Dongliang, Ju Guanqun
Urology Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Surgical Institute of Integrative Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Front Immunol. 2025 Apr 28;16:1543476. doi: 10.3389/fimmu.2025.1543476. eCollection 2025.
Prostate cancer (PCa) is a prevalent malignancy in men, and understanding its molecular mechanisms is crucial for identifying therapeutic targets.
Transcriptomic data from prostate tumors and matched healthy tissues were obtained from The Cancer Genome Atlas (TCGA). Differential expression analysis using the DESeq2 algorithm identified differentially expressed genes (DEGs). Cox proportional hazards regression was used to evaluate prognostic significance. Clinical validation involved comparing tumor specimens with normal tissues, focusing on BAIAP2L2, which showed significant differential expression and was further examined via immunohistochemical analysis. knockdown experiments were conducted in PC3 and DU145 cell lines to assess BAIAP2L2's functional role through assays for migration, colony formation, and proliferation.
A total of 1,449 DEGs were identified, including 775 upregulated and 674 downregulated genes. Prognostic analysis revealed 748 genes linked to clinical outcomes, with 19 hub genes identified. QPCR confirmed significant upregulation of four candidates, including BAIAP2L2, which was also elevated in malignant tissues. BAIAP2L2 knockdown significantly impaired migration, proliferation, and viability in PCa cells.
This study highlights crucial molecular mechanisms in PCa progression, particularly the significance of BAIAP2L2 as a potential therapeutic target, warranting further investigation into additional hub genes for effective targeted strategies.
前列腺癌（PCa）是男性中一种常见的恶性肿瘤，了解其分子机制对于确定治疗靶点至关重要。
从癌症基因组图谱（TCGA）获取前列腺肿瘤及匹配的健康组织的转录组数据。使用DESeq2算法进行差异表达分析，以识别差异表达基因（DEGs）。采用Cox比例风险回归评估预后意义。临床验证包括将肿瘤标本与正常组织进行比较，重点关注BAIAP2L2，其显示出显著差异表达，并通过免疫组织化学分析进一步检测。在PC3和DU145细胞系中进行敲低实验，通过迁移、集落形成和增殖测定来评估BAIAP2L2的功能作用。
共鉴定出1449个DEGs，其中775个基因上调，674个基因下调。预后分析显示748个基因与临床结局相关，确定了19个核心基因。QPCR证实了包括BAIAP2L2在内的四个候选基因显著上调，BAIAP2L2在恶性组织中也升高。敲低BAIAP2L2显著损害前列腺癌细胞的迁移、增殖和活力。
本研究突出了前列腺癌进展中的关键分子机制，特别是BAIAP2L2作为潜在治疗靶点的重要性，有必要进一步研究其他核心基因以制定有效的靶向策略。
