Chen Chong, Wang Min, Huang Tao, Huang Dong-Liang, Yu Shuai, Zhao Hui-Min, Fu Xiang-Xiang, Li Xin-Xin, Wu Hua
Department of Clinical Laboratory, Hainan General Hospital, Haikou, Hainan, China.
Department of Pharmacy, Hainan General Hospital (Hainan Medical University Hainan Hospital), Haikou, Hainan, China.
Front Cell Infect Microbiol. 2025 Apr 28;15:1560634. doi: 10.3389/fcimb.2025.1560634. eCollection 2025.
Rarely does occur in bloodstream infections (BSI), although it's typically found in cystic fibrosis. This study aims to decipher the genetic map and obtain insights of clinical symptoms into Pandoraea from BSI patients.
30 suspected BSI patients' diagnostic records and medical histories were recorded. spp. isolates were collected and subjected to antimicrobial susceptibility testing, Sanger sequencing and Whole-genome sequencing (WGS).
Of the 30 clinical cases, five (16.67%) ultimately died, whereas 25 (83.33%) are alive. 30 purified isolates showed high degree of MIC values to Meropenem, Amoxicillin and Potassium Clavulanate, Gentamicin, and Ceftazidime. Then, all isolates were identified as based on the 16S rRNA-based phylogenetic analysis. Among 28 genomes of them, the average genome size and average GC contents were 5,397,568 bp, and 62.43%, respectively. However, WP1 displayed high similarity (90.6%) to reference sp. LMG 31114. Genetic differences between the tested isolates and LMG 31114 suggested that the outbreak's causative pathogen could be a novel cluster of . The genomes accumulated mutations at an estimated rate of 1.3 × 10 mutations/year/site. Moreover, 26 clinical isolates within the cluster were formed in July 2014, revealing a tendency to develop regional endemic patterns.
BSI caused by this novel cluster of is linked to significant morbidity and mortality. Such cluster remains a critical public health challenge due to their regional epidemiological patterns and antibiotic treatment risk. This study contributed to the basis on pathogen identification, disease diagnosis, and BSI treatment.
虽然它通常在囊性纤维化患者中被发现，但在血流感染（BSI）中很少发生。本研究旨在解析潘多拉菌属的基因图谱，并深入了解BSI患者的临床症状。
记录30例疑似BSI患者的诊断记录和病史。收集潘多拉菌属分离株并进行药敏试验、桑格测序和全基因组测序（WGS）。
在30例临床病例中，5例（16.67%）最终死亡，而25例（83.33%）存活。30株纯化的潘多拉菌属分离株对美罗培南、阿莫西林和克拉维酸钾、庆大霉素和头孢他啶显示出高度的最低抑菌浓度值。然后，根据基于16S rRNA的系统发育分析，所有分离株均被鉴定为潘多拉菌属。在其中28个基因组中，平均基因组大小和平均GC含量分别为5,397,568 bp和62.43%。然而，WP1与参考潘多拉菌属菌株LMG 31114显示出高度相似性（90.6%）。测试分离株与LMG 31114之间的基因差异表明，此次疫情的致病病原体可能是潘多拉菌属的一个新集群。这些基因组以估计1.3×10⁻⁶突变/年/位点积累突变。此外，该潘多拉菌属集群内的26株临床分离株于2014年7月形成，显示出形成区域地方病模式的趋势。
由这种新型潘多拉菌属集群引起的BSI与显著的发病率和死亡率相关。由于其区域流行病学模式和抗生素治疗风险，此类集群仍然是一个关键的公共卫生挑战。本研究为病原体鉴定、疾病诊断和BSI治疗奠定了基础。
