Piliavskaia A S, Panchenko N E
Ukr Biokhim Zh (1978). 1985 Jul-Aug;57(4):65-7.
Bovine and human blood plasma contains alpha 2-antiplasmin which possesses affinity to lysin-binding sites in plasmin and inhibits the human plasmin. Its isolation was conducted for two stages: separation of plasminogen on lysin-cellulose, fractionation by ammonium sulphate, desalination on molselector G-25, chromatography on DEAE-Sephadex A-50 and affinity chromatography on plasmin-sepharose with the blocked active site.
牛和人血浆中含有α2-抗纤溶酶,它对纤溶酶中的赖氨酸结合位点具有亲和力,并能抑制人纤溶酶。其分离过程分两个阶段进行:在赖氨酸纤维素上分离纤溶酶原,用硫酸铵分级分离,在Molselector G-25上脱盐,在DEAE-葡聚糖A-50上进行色谱分析,以及在活性位点被封闭的纤溶酶-琼脂糖上进行亲和色谱分析。