Talha Moamen Saeed, Elshobary Mostafa E, Khairy Hanan M, Alprol Ahmed E
Agricultural and Biosystems Engineering Department, Faculty of Agriculture, Alexandria University, Alexandria, 21545, Egypt.
Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
Environ Sci Pollut Res Int. 2025 Jun;32(29):17407-17424. doi: 10.1007/s11356-025-36488-z. Epub 2025 May 13.
This study explored the potential of a newly isolated strain of Chlorella sorokiniana for dairy wastewater (DWW) phycoremediation and biodiesel production. Microalgae were cultivated in various dilutions of dairy wastewater (50%, 60%, 70%, 80%, and 90%) and compared with growth in synthetic Amaral medium. The results demonstrated that C. sorokiniana exhibited robust growth across all wastewater dilutions, with the 90% dilution outperforming the synthetic medium in terms of cell concentration and biomass production (0.47 g/L/d). Chlorophyll a content increased with higher wastewater concentrations, peaking at 20.24 µg/L in 90% DWW. Lipid content analysis revealed the highest accumulation in 90% DWW, reaching 40.33% of the dry weight biomass. Fatty acid profiling revealed a predominance of palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:2) across all treatments. The proportion of saturated fatty acids slightly increased with higher wastewater concentrations, while monounsaturated fatty acids decreased and polyunsaturated fatty acids remained relatively stable. The results indicated that moderate dilution of 60% wastewater resulted in the highest nitrate removal efficiency (75%). Phosphate removal remained consistently high (85-98%) across all dilutions. The biodiesel properties derived from wastewater-cultivated algae met the ASTM D6751 and EN14214 standards, with improvements in the cetane number and cloud point at higher wastewater concentrations. This study demonstrated the feasibility of using dairy wastewater as a cost-effective and sustainable medium for microalgal cultivation, offering the dual benefits of wastewater phycoremediation and high-quality biodiesel feedstock production. The findings highlight the potential for integrating algal cultivation with dairy industry waste management, contributing to circular economic principles in the biofuel sector.
本研究探索了新分离出的索氏小球藻菌株用于处理乳制品废水（DWW）及生产生物柴油的潜力。微藻在不同稀释度的乳制品废水（50%、60%、70%、80%和90%）中培养，并与在合成阿马拉尔培养基中的生长情况进行比较。结果表明，索氏小球藻在所有废水稀释度下均表现出强劲的生长态势，90%稀释度在细胞浓度和生物量产量（0.47克/升/天）方面优于合成培养基。叶绿素a含量随废水浓度升高而增加，在90%的乳制品废水中达到峰值20.24微克/升。脂质含量分析显示，90%的乳制品废水中脂质积累最高，达到干重生物量的40.33%。脂肪酸谱分析表明，所有处理中棕榈酸（C16:0）、油酸（C18:1）和亚油酸（C18:2）占主导地位。饱和脂肪酸的比例随废水浓度升高略有增加，而单不饱和脂肪酸减少，多不饱和脂肪酸相对稳定。结果表明，60%废水的适度稀释导致硝酸盐去除效率最高（75%）。在所有稀释度下，磷酸盐去除率一直保持在较高水平（85 - 98%）。从废水培养的藻类中提取的生物柴油性能符合ASTM D6751和EN14214标准，在较高废水浓度下十六烷值和浊点有所改善。本研究证明了将乳制品废水用作微藻培养的经济高效且可持续的培养基的可行性，兼具废水藻修复和高质量生物柴油原料生产的双重益处。研究结果突出了藻类培养与乳制品行业废物管理相结合的潜力，有助于生物燃料领域的循环经济原则。
