Li Hanyun, Liu Yinrui, Wang Mingyao, Xu Shaoshao, Liu Jilan, Qin Xianyun, Li Banban, Sun Jikui, Liu Yan, Jin Feng
Cheeloo College of Medicine, Shandong University, Jinan, China.
Xijing Hospital, Air Force Medical University, Xi'an, China.
Discov Oncol. 2025 May 13;16(1):753. doi: 10.1007/s12672-025-02284-x.
Fanconi anemia supplementation group I (FANCI), one of the Fanconi family proteins, may be closely related to the malignant progression of glioma cells. Here, we sought to validate the expression of FANCI in glioma cells and its role in regulating the Akt signaling pathway in the malignant progression of glioma cells.
The expression of FANCI in glioma cells was analyzed by bioinformatics and microarray. Lentivirus transfection was used to regulate the expression of FANCI in glioma cells. CCK-8, EdU, Transwell, and flow cytometry were used to observe the effect of FANCI on the malignant progression of glioma cells.
We found that low expression of FANCI inhibited the proliferation, migration and invasion of human glioma cells, and promoted cell apoptosis. However, overexpression of FANCI produced the opposite effect. We also found that low expression of FANCI inhibited the expression of P-Akt and B-cell lymphoma-2 (Bcl-2). Finally, we validated these in vitro results in a xenograft mouse model.
FANCI can inhibit the development of glioma by inhibiting Akt/Bcl-2 signaling pathway.
范可尼贫血补充组I（FANCI）是范可尼家族蛋白之一，可能与胶质瘤细胞的恶性进展密切相关。在此，我们试图验证FANCI在胶质瘤细胞中的表达及其在调节胶质瘤细胞恶性进展中Akt信号通路的作用。
通过生物信息学和微阵列分析胶质瘤细胞中FANCI的表达。利用慢病毒转染调节胶质瘤细胞中FANCI的表达。采用CCK-8、EdU、Transwell和流式细胞术观察FANCI对胶质瘤细胞恶性进展的影响。
我们发现FANCI低表达抑制人胶质瘤细胞的增殖、迁移和侵袭，并促进细胞凋亡。然而，FANCI过表达产生相反的效果。我们还发现FANCI低表达抑制P-Akt和B细胞淋巴瘤-2（Bcl-2）的表达。最后，我们在异种移植小鼠模型中验证了这些体外结果。
FANCI可通过抑制Akt/Bcl-2信号通路抑制胶质瘤的发展。
